Results 71 to 80 of about 2,262,054 (301)

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Solid phase extraction for removal of matrix effects in lipophilic marine toxin analysis by liquid chromatography-tandem mass spectrometry [PDF]

, 2009
The potential of solid phase extraction (SPE) clean-up has been assessed to reduce matrix effects (signal suppression or enhancement) in the liquid chromatography-tandem mass spectrometry (LC¿MS/MS) analysis of lipophilic marine toxins.
Boer, J., de, Bire, Ronel, Bire, R., de Boer, J., de Boer, J.; id_orcid, Jacob de Boer, Boer, J. de, Mulder, Patrick P. J., McElhinney, A. M., Hess, Philipp, McElhinney, M., Philipp Hess, Gerssen, A., Mairead A. McElhinney, Gerssen, Arjen, Ronel Bire, Arjen Gerssen, Bire, L., de Boer, Jacob, Patrick P. J. Mulder, Mulder, P.P.J., Hess, P., McElhinney, Mairead A.   +22 more
core   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Application of Liquid Chromatography in Food Analysis

Foods, 2020
Food products are very complex mixtures consisting of naturally-occurring compounds and other substances, generally originating from technological processes, agrochemical treatments, or packaging materials [...]
Oscar Núñez, Paolo Lucci
doaj   +1 more source

Modeling of Preparative Liquid Chromatography

, 2013
Preparative chromatography is today the best generic method for the purification of small drugs and valuable chemical components at the <10 kg-level. Recent progress in computer technology and the development of new nonchiral and chiral stationary
J. Samuelsson, P. Forssén, T. Fornstedt, Forssén, Patrik,, Samuelsson, Jörgen,, Fornstedt, Torgny,   +5 more
core   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Ionic liquids in liquid chromatography

Journal of Chromatography Open
The use of ionic liquids (ILs) in liquid chromatography (LC) is reviewed. ILs have several different types of functionalities, which means that they are prone to multiple simultaneous interactions with stationary phases in LC.
Sini Santasalo, Susanne K. Wiedmer
doaj   +1 more source

The Advancement of Narrow Open Tubular Liquid Chromatography [PDF]

, 2021
Open tubular columns are primarily used in gas chromatography, and they were regarded as the best choice to achieve high separation efficiency in gas chromatography. Substances diffuse much slower in liquids than in gases.
Xiang, Piliang
core