Results 231 to 240 of about 687,001 (274)

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

Is price associated with the quality of medicines? Evidence from active pharmaceutical ingredient testing in Nigeria. [PDF]

PLoS One
Montás MC, Anyakora C, Maffioli EM.
europepmc   +1 more source

F‐Box and Leucine‐Rich Repeat Protein 4 (FBXL4) Maintains Sarcomere Integrity and Cardiac Function by Enhancing K48‐Linked Ubiquitinated Degradation of Profilin‐1 (PFN1)

Advanced Science, EarlyView.
Schematic diagram depicting the proposed signaling mechanisms underlying the effects of FBXL4 in the setting of cardiac hypertrophy. Under hypertrophic stimulation, cardiomyocytes‐specific overexpression FBXL4 maintains sarcomere integrity and cardiac function by enhancing K48‐linked ubiquitinated degradation of PFN1 at the K70 site.
Xingda Li, Xueqi He, Xinyuan Hao, Yu Zhang, Xin Zhao, Shuang Wang, Zhenru Wang, Haonan Du, Hongda Li, Lian Yi, Zhimin Du, Weijie Du   +11 more
wiley   +1 more source

Double Fatal Sodium Nitrite Poisoning-Double Homicide, Extended Suicide, or Suicide and Accident? [PDF]

Int J Mol Sci
Smędra A, Wochna K, Lisowski M, Skulska-Birgiel A, Suchan M, Berent J.   +5 more
europepmc   +1 more source

Core Fucosylation Represses SMURF1‐Dependent Degradation of CD47 to Promote Tumor Immune Evasion

Advanced Science, EarlyView.
FUT8‐mediated core fucosylation of CD47 at N111 blocks SMURF1 binding and reduces CD47 ubiquitination and degradation. Blocking N111 glycosylation reduces CD47 expression and promotes macrophage phagocytosis of tumor cells. Furthermore, ablating CD47 core fucosylation boosts CD103+ dendritic cells (DCs) infiltration, increases natural killer (NK) cell ...
Yuting Cao, Siyuan Chai, Mingyang Li, Xiaoming Chen, Jiating Hu, Bingyi Lin, Liming Wu, Wen Yi, Qiang Zhu   +8 more
wiley   +1 more source

Alphaviral Capsid Proteins Inhibit Stress Granule Assembly via Competitive RNA Binding With G3BP1

Advanced Science, EarlyView.
Stress granules exert antiviral functions. This study illustrates a conserved function of alphaviral capsid proteins in modulating stress granules. Oligomerization mediated by a helical motif coupled with a positively charged intrinsically disordered region (IDR) directly competes with G3BP1 for RNA binding, thereby disrupting G3BP1‐RNA liquid–liquid ...
Yun Zhang, Yi Liu, Zhiying Yao, Haolong Lai, Xiaoxin Chen, Ziqiu Wang, Yiqiong Bao, Tingting Li, Xiaoming Zhou, Xiabin Chen, Peiguo Yang   +10 more
wiley   +1 more source

Synthesis of 4'-C-α-Aminoethoxy-2'-O-Methyl-5-Methyl- and 5-Propynyl-Uridine Phosphoramidites for Structurally Optimized Antisense Oligonucleotides. [PDF]

Curr Protoc
Mahmoud EM, Ueno Y.
europepmc   +1 more source

Dataset on phytochemical profiling of <i>Moringa oleifera</i> leaves ethanolic extract using spectrophotometry UV-Vis, TLC, FTIR, and GCMS: Neuroregenerative potential, IC₅₀ determination, and proliferation assay evaluation. [PDF]

Data Brief
Sanjaya WBT, Widayanti R, Nishijima K, Wihadmadyatami H, Kustiati U, Aliffia D, Aviana AP, Kusindarta DL.   +7 more
europepmc   +1 more source

<i>Actinidia arguta</i>: Biological and Health Promoting Properties-Analysis of Bioactive Components. [PDF]

Plants (Basel)
Choma IM, Olszowy-Tomczyk M.
europepmc   +1 more source

Development and Validation of RP-HPLC Method for <i>trans</i>-Resveratrol in HPβCD-Loaded Stealth Liposomes: Stability and Release Studies. [PDF]

ACS Omega
Marwa A, Bahtiar A, Shamsuddin AF, Sari K, Syarif M, Restu WK, Jufri M.   +6 more
europepmc   +1 more source