Results 51 to 60 of about 687,001 (274)

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Paper Chromatography for the Radiochemical Control of 90Y for it’s Clinical Uses [PDF]

open access: yesمجله علوم و فنون هسته‌ای, 2013
Our aim in this work was to develop a reliable technique to accurately determine the amount of Yttrium-90 in Strontium-90 used for therapy. Yttrium-90 which can be used for clinical purposes should be in a very high radionuclide purity,therefore a ...
A.R Khanchi   +4 more
doaj  

Assay of nanogram levels of triglyceride lipase with a radioactive substrate

open access: yesJournal of Lipid Research, 1972
A simple, sensitive procedure for the determination of triglyceride lipase activity has been developed. Nanogram amounts of oleic acid hydrolyzed from commercially available [14C]triolein were readily determined by the counting of the radioactivity of ...
Walton H. Marsh, Patrick J. Fitzgerald
doaj   +1 more source

Impact of Acid Rain with Different pH Values in Monstera deliciosa Plants

open access: yesMedical Sciences Forum, 2023
The present research experimentally outlines the reaction of a Monstera deliciosa plants to the effects of acid rain at various pH levels. Sulfuric acid was used to simulate acid rain, with prepared solutions having four different pH values.
Afonso Ramos   +4 more
doaj   +1 more source

A field deployable method for a rapid screening analysis of inorganic arsenic in seaweed [PDF]

open access: yes, 2017
The authors thank the support for getting the seaweed samples from the projects funded under the Department of Agriculture, Food and the Marine’s Competitive research programmes in Ireland. Reference number 14 SF 860. The authors thank Corny Brombach for
Bralatei, Edi   +7 more
core   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Compendio bibliográfico sobre cromatografía líquida de alta eficacia de lípidos: IV 1985-1994.

open access: yesGrasas y Aceites, 1997
The present summary, following the tradition begun by K. Aitzetmüller with the works published from 1961 and continued by the present authors from 1975, is a compilation of articles appearing between the years given in the title; the database used was ...
E. Graciani Constante   +2 more
doaj   +1 more source

The chromatographic identification of some biologically important phosphate esters [PDF]

open access: yes, 1951
the objective of the present work was to provide a means for separating and indentifying phosphate esters involved in glycolysis in higher plants. Paper chromatography of phosphate esters has been employed by several workers, most notably Benson et al ...
Axelrod, Bernard, Bandurski, Robert S.
core  

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

Multidimensional Chromatography and Its Applications in Food Products, Biological Samples and Toxin Products: A Comprehensive Review

open access: yesSeparations, 2022
Food, drugs, dyes, extracts, and minerals are all made up of complex elements, and utilizing unidimensional chromatography to separate them is inefficient and insensitive.
Ruhul Amin   +6 more
doaj   +1 more source

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