ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Mobile Image Ratiometry: A New Method for Instantaneous Analysis of Rapid Test Strips [PDF]
Here we describe Mobile Image Ratiometry (MIR), a new method for the automated quantification of standardized rapid immunoassay strips using consumer-based mobile smartphone and tablet cameras.
Bryan Callahan +3 more
core +2 more sources
Single-step isolation of extracellular vesicles by size-exclusion chromatography
Background Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of ...
A. Böing +5 more
semanticscholar +1 more source
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu +5 more
wiley +1 more source
Detection strategies for bioassays based on liquid chromatography, fluorescence spectroscopy and mass spectrometry [PDF]
New detection strategies for bioassays based on liquid chromatography,\ud fluorescence spectroscopy and mass spectrometry were developed and are\ud presented within this ...
Hempen, Christel Maria
core +1 more source
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Isolation of amyloidogenic aggregates of transthyretin by ceramic hydroxyapatite chromatography
Amyloidogenic proteins can lapse into unstable monomeric precursors which misfold and self-assemble into toxic oligomers and amyloid aggregates leading to intracellular deposition of insoluble amyloid fibrils, the hallmark of amyloidosis and major ...
Maria K. Tsoumpra +2 more
doaj +1 more source
Lipidomic approach to the analysis of Persea americana Mill. cv. from the northern subregion of the department of Caldas [PDF]
Aim: Colombia’s economy relies heavily on agriculture, with Hass avocado (Persea americana) playing a key role. This fruit consists of exocarp, mesocarp, and endocarp, with the mesocarp being the most consumed part due to its rich composition of ...
Juan Pablo Betancourt Arango +5 more
doaj +1 more source
Apple scab control with grapefruit seed extract: no alternative to chemical fungicides [PDF]
The growth inhibiting effect of four commercially available grapefruit seed extracts on the causal organism of apple scab Venturia inaequalis was tested.
Kelderer, Markus +5 more
core
Newborn screening by liquid chromatography-tandem mass spectrometry: A future perspective in India
Banerjee Abhik, Sil Rajarshi
openalex +1 more source

