Results 91 to 100 of about 1,589,585 (300)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Chromatography : fundamentals and applications of chromatography and related differential migration methods.

, 1992
Includes bibliographical references and indexes.Print version record.Front Cover; Chromatography, 5th edition: Applications; Copyright Page; Contents; List of Authors; List of Abbreviations; List of Italic Symbols; List of Greek Symbols; Chapter 12 ...
Heftmann, Erich.
core  

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Isolation of amyloidogenic aggregates of transthyretin by ceramic hydroxyapatite chromatography

Journal of Chromatography Open
Amyloidogenic proteins can lapse into unstable monomeric precursors which misfold and self-assemble into toxic oligomers and amyloid aggregates leading to intracellular deposition of insoluble amyloid fibrils, the hallmark of amyloidosis and major ...
Maria K. Tsoumpra, Shintaro Kobayashi, Eri Chatani   +2 more
doaj   +1 more source

Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry [PDF]

, 2005
A convenient liquid chromatography-tandem mass spectrometry method for the quantification of the triazole antifungal agent voriconazole in plasma samples is described. Fenbuconazole is used as an internal standard.
Spöhrer, Ute, Schiel, Xaver, Vogeser, Michael   +2 more
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Determination of trace amounts of carboxylic acids in ambient air by capillary gas chromatography - mass spectrometry

, 2022
S.297-304A method for the determination of organic acids in ambient air using an ion-exchange resin in a bifunctional manner is presented. The ion-exchange resin is used as an adsorbent for sampling and subsequently as catalyst for the methylation of the
Sollinger, S., Levsen, K., Emmrich, M.
core   +1 more source

Seasonal Variation of the Chemical Composition and Antimicrobial and Cytotoxic Activities of the Essential Oils from Inga laurina (Sw.) Willd.

Molecules, 2014
The seasonal chemical composition of essential oils from Inga laurina was determined by GC/MS. In the stem bark’s essential oil extracted during the dry season, the presence of terpenoids (30.05%) stood out, and phytol (9.76%) was the major compound ...
Fabiana B. Furtado, Francisco J. T. de Aquino, Evandro A. Nascimento, Carla de M. Martins, Sérgio A. L. de Morais, Roberto Chang, Luís C. S. Cunha, Luís F. Leandro, Carlos H. G. Martins, Mário M. Martins, Claudio V. da Silva, Fabrício C. Machado, Alberto de Oliveira   +12 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Current chromatographic methods to determine cannabinoids in biological samples: A review of the state-of-the art on sample preparation techniques

Green Analytical Chemistry
Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) are the primary compounds of interest in the plant cannabis sativa, used mainly as recreational drug.
Igor Gustavo Carvalho Oliveira, Caroline Fernandes Grecco, Israel Donizeti de Souza, Maria Eugênia Costa Queiroz   +3 more
doaj   +1 more source