Results 1 to 10 of about 1,193,888 (281)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies. [PDF]

FEBS Open Bio
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Janssen B, Romein R, van den Berg WAM, Lindhoud S, Borst JW, van Mierlo CPM.   +5 more
europepmc   +2 more sources

A Novel Recombinant Protein Purification Approach Using Biomolecular Condensates. [PDF]

Int J Mol Sci
Fu Y, Zhang H.
europepmc   +1 more source

Development of single-chain C1q affinity chromatography-mass spectrometry for the glycoform-resolved characterization of low-affinity immunoglobulin G interactions. [PDF]

MAbs
Lippold S, Chang K, Dürkoop M, Andersen JT, Yang F, Schlothauer T.   +5 more
europepmc   +1 more source

Mammalian Arachidonic Acid 15-Lipoxygenases: Fed-Batch Fermentation, Enzyme Purification and Functional Characterization. [PDF]

Metabolites
Aksenov V, Kurchatova AV, Golovanov A, Ulasenko V, Zubkova O, Zhuravlev A, Makishvili E, Kushlinskii NE, Kuhn H, Ivanov I.   +9 more
europepmc   +1 more source

One-step cleavage and purification: a bifunctional IdeS-Protein A fusion protein for on-column generation of antibody fragments. [PDF]

MAbs
Zhang T, Liao H, Teng CB, Wang C.
europepmc   +1 more source

Advances in Downstream Processing of Monoclonal Antibodies. [PDF]

Antibodies (Basel)
Kołodziej M, Antos D.
europepmc   +1 more source

Fluorescent Detection of Aqueous <i>N</i>-Nitrosodimethylamine via Photochemical Transformation and Affinity Capture. [PDF]

J Am Chem Soc
Wang CH, Hung HM, Croy RG, Essigmann JM, Pentelute BL, Swager TM.   +5 more
europepmc   +1 more source

Bioprocess Development of a Human Monoclonal Antibody against MERS-CoV: Analytical and Preclinical Studies. [PDF]

Biomol Ther (Seoul)
Lim J, Lee H, Lee JY, Jo DG, Hong S, Lee SY, Park BK, Jeong Y, Seo J, Park S, Jung H, Park CK, Kim KC, Cho I.   +13 more
europepmc   +1 more source

Innovative Test Strip-Based Colorimetric Sensors Integrated With Affinity Chromatography: Acetylcholinesterase Inhibitor Screening Breakthrough in Lycium Barbarum Leaves. [PDF]

Adv Sci (Weinh)
Sa Y, Yuan H, Ma J, Yang Z, Wang M, Wang W, Yang L, Ma F, Zhang W, Mening'oo GW, Chen G, Ma X.   +11 more
europepmc   +1 more source

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice. [PDF]

Biotechnol Bioeng
Lu S, Setua S, Khan MA, Bansal A, O'Boyle Q, Cabrales P, Irwin DC, Buehler PW, Palmer AF.   +8 more
europepmc   +1 more source