Results 11 to 20 of about 333,536 (310)

Weak-affinity chromatography

open access: yesNature, 1990
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations. 
D. Zopf   +3 more
core   +2 more sources

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

open access: yesmAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall   +6 more
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

open access: yesPharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes   +5 more
doaj   +1 more source

Screening Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry

open access: yes, 2021
Protein overexpression and purification are critical for in vitro structure-function characterization studies. However, some proteins are difficult to express robustly in heterologous systems due to host-related (e.g., codon usage, translation rate) and ...
Vicki, Wysocki   +4 more
core   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

open access: yesQuímica Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki   +3 more
doaj   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

open access: yesEnvironmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

open access: yesBiomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

open access: yesSensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne   +2 more
doaj   +1 more source

Cryogels with Affinity Ligands as Tools in Protein Purification

open access: yes, 2015
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract.
Bo Mattiasson   +4 more
core   +1 more source

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

open access: yesCzech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco   +5 more
doaj   +1 more source

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