Results 11 to 20 of about 529,464 (310)

Interaction of the 89K murine cytomegalovirus immediate-early protein with core histones [PDF]

open access: yes, 1988
The conditions that permit the interaction of immediate-early proteins of murine cytornegalovirus (MCMV) with DNA were studied. Chromatography of extracts from infected cells on MCMV DNA cellulose and calf thymus DNA cellulose showed that pp89, the ...
Alberts   +32 more
core   +1 more source

Effects of antibodies against tubulin on the movement of reactivated sea urchin sperm flagella [PDF]

open access: yes, 1980
Antibodies binding to sea urchin flagellar outer-doublet tubulin have been isolated from rabbit sera by tubulin-affinity chromatography employing electrophoretically purified tubulin as the immobilized substrate.
Asai, David J., Brokaw, Charles J.
core   +1 more source

Purification of baculovirus vectors using heparin affinity chromatography

open access: yesMolecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman   +3 more
doaj   +1 more source

Enhanced production of recombinant HALT-1 pore-forming toxin using two-step chromatographic procedure

open access: yesMethodsX, 2023
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap   +3 more
doaj   +1 more source

The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]

open access: yes, 1993
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Daniel, Roy M.   +3 more
core   +2 more sources

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

open access: yesmAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall   +6 more
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

open access: yesPharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes   +5 more
doaj   +1 more source

A Monomer-to-Trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) Is Associated with a Loss of in Vitro Activity [PDF]

open access: yes, 2004
The human mitochondrial transcription termination factor (mTERF) is a nuclear-encoded 39-kDa protein that recognizes a mtDNA segment within the mitochondrial tRNALeu(UUR) gene immediately adjacent to and downstream of the 16 S rRNA gene. Binding of mTERF
Asin-Cayuela, Jordi   +2 more
core   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

open access: yesQuímica Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki   +3 more
doaj   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

open access: yesEnvironmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

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