Results 31 to 40 of about 1,255,564 (310)

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

open access: yesBiomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

open access: yesSensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne   +2 more
doaj   +1 more source

High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography

open access: yesMolecular Therapy: Methods & Clinical Development, 2023
The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea   +8 more
doaj   +1 more source

Cryogels with Affinity Ligands as Tools in Protein Purification

open access: yes, 2015
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract.
Bo Mattiasson   +4 more
core   +1 more source

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

open access: yesCzech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco   +5 more
doaj   +1 more source

High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography

open access: yesScientific Reports, 2021
Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti   +4 more
doaj   +1 more source

PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography

open access: yesActa Scientiarum: Biological Sciences, 2016
Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo   +5 more
doaj   +1 more source

Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines

open access: yes, 2007
A stationary phase for high performance affinity chromatography with immobilization of DNA onto silica gel was prepared and characterized. The effect of the ionic strength, concentration of Mg2+, EDTA and CH3 CN in the mobile phase on the retention of ...
Hu, Lianghai   +5 more
core   +1 more source

Recent developments in quantitative affinity chromatography

open access: yes, 1992
This review surveys developments during the past decade in the use of quantitative affinity chromatography as a means of evaluating equilibrium constants for solute-ligand and solute-matrix interactions.
Winzor D.J., Donald J. Winzor
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

open access: yesFEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang   +4 more
wiley   +1 more source

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