High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography
Molecular Therapy: Methods & Clinical Development, 2023 The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea +8 more
doaj +1 more source
Cryogels with Affinity Ligands as Tools in Protein Purification
, 2015 Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract.
Bo Mattiasson +4 more
core +1 more source
Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography
Sensors, 2017 l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne +2 more
doaj +1 more source
High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
doaj +1 more source
PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography
Acta Scientiarum: Biological Sciences, 2016 Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo +5 more
doaj +1 more source
Removal of dsRNA byproducts using affinity chromatography
Molecular Therapy: Nucleic AcidsDouble-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark +8 more
doaj +1 more source
Purification of phage display-modified bacteriophage T4 by affinity chromatography
BMC Biotechnology, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
doaj +1 more source
Effect of Q Chromatography on the Recovery of Human Plasminogen in Affinity Chromatography
Zhongguo shuxue zazhi[Objective] To compare quality control (relative purity and specific activity) and process control [plasminogen (Pg) antigen recovery and potency recovery] indexes of samples before and after adding the Q chromatography step to the full chromatography ...
YUE Shenglan +12 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns. [PDF]
PLoS ONE, 2017 Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role ...
Anna Katharina Weyrauch +4 more
doaj +1 more source