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Molecular Biotechnology, 2000
Immunoaffinity chromatography is a process in which the binding affinity of an antigen to a parent antibody is utilized as a basis of separation. Owing to the customized avidity and specificity, monoclonal antibodies (Mabs) have become indispensable for both protein characterization and purification.
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Immunoaffinity chromatography is a process in which the binding affinity of an antigen to a parent antibody is utilized as a basis of separation. Owing to the customized avidity and specificity, monoclonal antibodies (Mabs) have become indispensable for both protein characterization and purification.
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Chromatography and the chromatography discussion group
TrAC Trends in Analytical Chemistry, 1982Abstract This year sees the 25th Anniversary of the founding of the Gas Chromatography Discussion Group which became, in 1972, the Chromatography Discussion Group. To mark the occasion, the 14th International Symposium on Chromatography was held in London in September. In this article Dr G.A.P.
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Current Protocols in Protein Science, 1996
AbstractThis unit describes the isolation of soluble or membrane‐bound protein antigens from cells or homogenized tissue by immunoaffinity chromatography. This technique involves the elution of a single protein from an immunoaffinity column after prior elution of nonspecifically adsorbed proteins.
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AbstractThis unit describes the isolation of soluble or membrane‐bound protein antigens from cells or homogenized tissue by immunoaffinity chromatography. This technique involves the elution of a single protein from an immunoaffinity column after prior elution of nonspecifically adsorbed proteins.
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Chromatography: High-Performance Liquid Chromatography
2016High-performance liquid chromatography (HPLC) is a major analytic tool in contemporary science, with possibly the highest number of systems installed and running globally. Modern HPLC offers high resolutions allowing the quantitative determination of target analytes within complex matrices by its compatibility with a number of detectors.
Gika, Helen +3 more
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1999
Abstract The principle of immunoaffinity or immunoadsorption chromatography is based on the highly specific interaction of an antigen with its antibody (1). Immunoaffinity chromatography is a specialized form of affinity chromatography and as such utilizes a ligand (either antigen or antibody) immobilized onto a solid support matrix in a
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Abstract The principle of immunoaffinity or immunoadsorption chromatography is based on the highly specific interaction of an antigen with its antibody (1). Immunoaffinity chromatography is a specialized form of affinity chromatography and as such utilizes a ligand (either antigen or antibody) immobilized onto a solid support matrix in a
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Latest Trends on the Future of Three-Dimensional Separations in Chromatography
Chemical Reviews, 2021Noor Abdulhussain +2 more
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