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Stress-Free Chromatography: Affinity Chromatography

Current Pharmaceutical Biotechnology, 2009
A number of approaches are available in minimizing aggregation of the final protein products. This chapter describes one such approach, i.e., an attempt to avoid stressful conditions that may eventually lead to protein aggregation. Affinity chromatography uses specific interaction between protein to be purified and ligand attached to the column. Due to
Tsutomu, Arakawa   +3 more
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Chromatography+RECOVERY=superresolution chromatography

Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2003
Abstract A method for improving the resolution of the chromatographic analysis based on deriving the point-spread function of a chromatographic column, i.e., a chromatogram of an individual compound, is described. The system of two data sets, namely, a chromatogram of a substance analyzed and a point-spread function of a chromatographic column in ...
E.L Kosarev, K.O Muranov
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Immunoaffinity chromatography

Molecular Biotechnology, 1994
The basic procedure of immunoaffinity chromatography (IAC) is described. The insoluble support matrices available for IAC and their activation chemistries, including some of the most recently introduced, are reviewed. Means of selecting the most appropriate monoclonal antibody (MAb) are described, although an empirical approach is still required for ...
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Chromatography

Introduction to Forensic Chemistry, 2018
Nadolol is a pharmaceutical chiral drug worldwide prescribed to the relief of some diseases mainly related with the cardiovascular system. Although some studies refer that the therapeutic effect of this drug is related with only one enantiomer, nadolol ...
K. M. Elkins
semanticscholar   +1 more source

Immunoaffinity Chromatography

1999
Abstract The principle of immunoaffinity or immunoadsorption chromatography is based on the highly specific interaction of an antigen with its antibody (1). Immunoaffinity chromatography is a specialized form of affinity chromatography and as such utilizes a ligand (either antigen or antibody) immobilized onto a solid support matrix ...
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Catalytic Chromatography

Analytical Biochemistry, 2000
Catalytic chromatography exploits both specific biological affinity and catalytic specificity to selectively purify enzymes. Two different applications are presented. Purification of EcoRI restriction endonuclease to apparent homogeneity was accomplished in a single step with significantly greater yield and purification than was obtained with affinity ...
L A, Jurado, J T, Drummond, H W, Jarrett
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Hydroxylapatite Chromatography

Current Protocols in Protein Science, 1997
AbstractHydroxylapatite (also called hydroxyapatite), a form of calcium phosphate, can be used as a matrix for the chromatography of both proteins and nucleic acids. Protocols are provided for both standard low‐pressure chromatography of a protein mixture using a hydroxylapatite column prepared in the laboratory, and an HPLC method, applicable to ...
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Advances in Chromatography

, 2017
Give us 5 minutes and we will show you the best book to read today. This is it, the advances in chromatography that will be your best choice for better reading book. Your five times will not spend wasted by reading this website.

semanticscholar   +1 more source

Immunoaffinity Chromatography

Current Protocols in Protein Science, 1996
AbstractThis unit describes the isolation of soluble or membrane‐bound protein antigens from cells or homogenized tissue by immunoaffinity chromatography. This technique involves the elution of a single protein from an immunoaffinity column after prior elution of nonspecifically adsorbed proteins.
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Green chromatography

Journal of Chromatography A, 2013
Analysis of organic compounds in samples characterized by different composition of the matrix is very important in many areas. A vast majority of organic compound determinations are performed using gas or liquid chromatographic methods. It is thus very important that these methods have negligible environmental impact.
Justyna, Płotka   +5 more
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