Results 51 to 60 of about 2,248,751 (362)

Analyzes of β-lactam antibiotics by direct injection of environmental water samples into a functionalized graphene oxide-silica packed capillary extraction column online coupled to liquid chromatography tandem mass spectrometry

open access: yesTalanta Open, 2023
An environmentally friendly and high-throughput method for monitoring β-lactam antibiotics in environmental water samples is presented. In this study, an in-lab synthesized graphene oxide supported onto aminopropyl silica (GO@SiO2) sorbent was packed ...
Edvaldo V.S. Maciel   +2 more
doaj  

Recent trends and developments in pyrolysis-gas chromatography: review [PDF]

open access: yes, 2008
Pyrolysis-gas chromatography (Py-GC) has become well established as a simple, quick and reliable analytical technique for a range of applications including the analysis of polymeric materials.
Arias   +179 more
core   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

open access: yesFEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt   +8 more
wiley   +1 more source

Influence of Selected Factors on Antioxidant Properties of Chlorogenic Acid and Kaempferol as Well as Their Binary Mixtures

open access: yesApplied Sciences, 2023
Plants are a rich source of substances with antioxidant properties, including high amounts of polyphenolic compounds, as well as other substances that do not necessarily have antioxidant properties.
Małgorzata Olszowy-Tomczyk   +1 more
doaj   +1 more source

Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects [PDF]

open access: yes, 2005
A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S ...
Butterwith   +10 more
core   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

open access: yesFEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito   +6 more
wiley   +1 more source

In vitro biomimetic HPLC and in vivo characterisation of GM6, an endogenous regulator peptide drug candidate for amyotrophic lateral sclerosis

open access: yesADMET and DMPK, 2018
Amyotrophic lateral sclerosis (ALS) is an idiopathic, fatal neurodegenerative disease of the human motor system. Subunits of the 33-amino acid containing motorneurontrophic factor (MNTF) have been investigated and GM6 has been found as a potential ...
Klara Livia Valko   +3 more
doaj   +1 more source

Selection of imprinted nanoparticles by affinity chromatography [PDF]

open access: yes, 2009
Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography.
Chianella, Iva   +4 more
core   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

open access: yesFEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez   +17 more
wiley   +1 more source

Application of biomimetic HPLC to estimate lipophilicity, protein and phospholipid binding of potential peptide therapeutics

open access: yesADMET and DMPK, 2018
Peptide therapeutics are new modalities offering several challenges to drug discovery. They are generally less stable and permeable in vivo. The characterization of their lipophilicity cannot be carried out using the traditional in silico or wet octanol ...
Klara Livia Valko   +4 more
doaj   +1 more source

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