Results 51 to 60 of about 2,091,143 (316)

Immobilization of cytochrome P450 enzymes onto magnetic beads: An approach to drug metabolism and biocatalysis

Talanta Open, 2023
Cytochrome P450 (CYP, P450) presents a wide range of applicability in drug metabolism studies and in biocatalysis as a great alternative to synthesizing compounds. Nonetheless, their lack of stability is one of their major drawbacks.
Izadora Liranço Furlani, Regina Vincenzi Oliveira, Quezia Bezerra Cass   +2 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Development and Validation of a Novel LC-MS/MS Method for a TDM-Guided Personalization of HSCT Conditioning with High-Dose Busulfan in Children

Biomedicines, 2023
Personalization of busulfan (Bu) exposure via therapeutic drug monitoring (TDM) is recommended for patients treated with high-dose conditioning regimens.
Alessia Cafaro, Federica Pigliasco, Giammarco Baiardi, Sebastiano Barco, Manuela Stella, Roberto Bandettini, Francesca Mattioli, Maura Faraci, Giuliana Cangemi   +8 more
doaj   +1 more source

Toxicological Analysis of Some Drugs of Abuse in Biological Samples [PDF]

, 2015
Consumption of drugs of abuse is a scourge of modern world. Abuse, drug addiction and their consequences are one of the major current problems of European society because of the significant repercussions in individual, family, social and economic level ...
Baconi, Daniela Luiza, Bârcă, Maria, Bălălău, Cristian, Ciobanu, Anne Marie, Negrei, Carolina, Stan, Miriana   +5 more
core   +3 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography [PDF]

, 2017
In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine
Cornely, Dr. Kathleen, Foley, Caroline
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Application of biomimetic HPLC to estimate lipophilicity, protein and phospholipid binding of potential peptide therapeutics

ADMET and DMPK, 2018
Peptide therapeutics are new modalities offering several challenges to drug discovery. They are generally less stable and permeable in vivo. The characterization of their lipophilicity cannot be carried out using the traditional in silico or wet octanol ...
Klara Livia Valko, Gabriela Ivanova-Berndt, Paul Beswick, Mark Kindey, Dorothy Ko   +4 more
doaj   +1 more source

Combining ion chromatography with mass spectrometry and inductively coupled plasma‐mass spectrometry: Annual review 2020

Analytical Science Advances, 2021
The demand for analyzing low molecular weight polar and ionic components in body fluids, pharmaceutical formulations, food, environmental samples, and drinking water is increasing. Ion chromatography (IC) offers significant advantages over RPLC and HILIC
Cees Bruggink, Detlef Jensen
doaj   +1 more source

Recent trends and developments in pyrolysis-gas chromatography: review [PDF]

, 2008
Pyrolysis-gas chromatography (Py-GC) has become well established as a simple, quick and reliable analytical technique for a range of applications including the analysis of polymeric materials.
Arias, Armitage, Aslan, Asperger, Asperger, Asperger, Asperger, Bertini, Beyer, Blackledge, Blokker, Bonaduce, Bruck, Buckley, Buco, Campbell, Casas-Catalán, Causin, Challinor, Challinor, Challinor, Chang, Chiavari, Choi, Choi, Colombini, De Koeijer, De la Cruz-Canizares, Dignac, Doménech-Carbó, Doménech-Carbó, Dunn, Dzierzega-Lecznar, Dzierzega-Lecznar, Egan, Erickson, Evans, Evans, Fabbri, Fabbri, Fanter, Fifield, Flodin, Garcette-Lepecq, Gleixner, González-Vila, Goodacre, Greenwood, Greenwood, Greenwood, Greenwood, Guo, Gupta, Göbbels, Gözet, Halket, Halket, Heffenden, Herrera, Horrocks, Ikeya, Ishikawa, Islas, Jakab, Jamin, Jansson, Jarde, Jia, Jin, Jose Gonzalez-Rodriguez, Karina L. Sobeih, Kechang, Kochanowski, Kumooka, Lattimer, Lazaro, Learner, Levy, Li, Ling, Liu, Mao, Marinari, Marinari, Mark Baron, Marques, Maynard, Meruva, Metz, Moldoveanu, Mowry, Munson, Mäkinen, Nierop, Odermatt, Odermatt, Oguz, Ohshima, Osete-Cortina, Osete-Cortina, Osete-Cortina, Park, Parsi, Parsi, Parsi, Parsi, Partouche, Pasch, Peacock, Pel, Pel, Perng, Perng, Petsch, Poerschmann, Poirier, Prasad, Prati, Raghavan, Raulin, Ristau, Ristolainen, Rodriguez, Ross, Räisänen, Sakayanagi, Scalarone, Schmidt, Schwarzinger, Sephton, Sessions, Snyder, Stauffer, Stout, Stout, Stout, Suelves, Sugden, Sun, Sundarrajan, Suuronen, Takayasu, Takayasu, Tanczos, Thomsen, Tsai, Tsuge, Tsuge, Tzamtzis, Urakami, Uyar, Uyar, Vanderborgh, Volkman, Wampler, Wampler, Wampler, Wang, Wang, Wang, Wang, Wang, Wang, Wang, Wang, Wang, White, Wilkes, Wilkes, Yamamoto, Yang, Yaylayan, Yinon, Zhang, Zhou, Zhu, Zimmerman, Zlotnick, Çoban-Yildiz, Łakomy   +179 more
core   +1 more source