Results 41 to 50 of about 1,712,951 (299)

Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery

Scientific Reports, 2022
Homology-directed repair-mediated knock-in (HDR-KI) in combination with CRISPR-Cas9-mediated double strand break (DSB) leads to high frequency of site-specific HDR-KI. While this characteristic is advantageous for generating genetically modified cellular
Kyotaro Yamazaki, Kyosuke Matsuo, Akane Okada, Narumi Uno, Teruhiko Suzuki, Satoshi Abe, Shusei Hamamichi, Nanami Kishima, Shota Togai, Kazuma Tomizuka, Yasuhiro Kazuki   +10 more
doaj   +1 more source

Condensin-Driven Remodeling of X-Chromosome Topology during Dosage Compensation

Nature, 2015
The three-dimensional organization of a genome plays a critical role in regulating gene expression, yet little is known about the machinery and mechanisms that determine higher-order chromosome structure.
Emily Crane, Qian Bian, R. McCord, B. Lajoie, Bayly S. Wheeler, E. Ralston, S. Uzawa, J. Dekker, B. Meyer   +8 more
semanticscholar   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

Chromosome-scale genome assembly of a natural diploid kiwifruit (Actinidia chinensis var. deliciosa) [PDF]

, 2023
Hui Xia, Honghong Deng, Mingzhang Li, Yue Xie, Lijin Lin, Huifen Zhang, Xian Luo, Xiulan Lv, Jin Wang, Dong Liang   +9 more
openalex   +1 more source

A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival

Molecular Oncology, EarlyView.
There is an unmet need in metastatic breast cancer patients to monitor therapy response in real time. In this study, we show how a noninvasive and affordable strategy based on sequencing of plasma samples with longitudinal tracking of tumour fraction paired with a statistical model provides valuable information on treatment response in advance of the ...
Emma J. Beddowes, Mario Ortega Duran, Solon Karapanagiotis, Alistair Martin, Meiling Gao, Riccardo Masina, Ramona Woitek, James Tanner, Fleur Tippin, Justine Kane, Jonathan Lay, Anja Brouwer, Stephen‐John Sammut, Suet‐Feung Chin, Davina Gale, Dana W. Y. Tsui, Sarah‐Jane Dawson, Nitzan Rosenfeld, Maurizio Callari, Oscar M. Rueda, Carlos Caldas   +20 more
wiley   +1 more source

Cdt1 stabilizes an open MCM ring for helicase loading

Nature Communications, 2017
The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading ...
Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley   +9 more
doaj   +1 more source

Joint control of meiotic crossover patterning by the synaptonemal complex and HEI10 dosage

Nature Communications, 2022
During meiosis, the number and distribution of crossovers (COs) are tightly controlled, but the mechanistic basis of this control is unclear. Here, by combining experimental data and mathematical modeling, the study advocates a CO patterning model via ...
Stéphanie Durand, Qichao Lian, Juli Jing, Marcel Ernst, Mathilde Grelon, David Zwicker, Raphael Mercier   +6 more
doaj   +1 more source

Single cell Hi-C reveals cell-to-cell variability in chromosome structure

Nature, 2013
Large-scale chromosome structure and spatial nuclear arrangement have been linked to control of gene expression and DNA replication and repair. Genomic techniques based on chromosome conformation capture (3C) assess contacts for millions of loci ...
T. Nagano, Yaniv Lubling, T. Stevens, S. Schoenfelder, E. Yaffe, W. Dean, E. Laue, A. Tanay, P. Fraser   +8 more
semanticscholar   +1 more source

Multi‐omic characterization of consensus molecular subtype 1 (CMS1) colorectal cancer with dampened immune response improves precision medicine

Molecular Oncology, EarlyView.
This study highlights the importance of multi‐omic analyses in characterizing colorectal cancers. Indeed, our analysis revealed a rare CMS1 exhibiting dampened immune activation, including reduced PD‐1 expression, moderate CD8+ T‐cell infiltration, and suppressed JAK/STAT pathway.
Livia Concetti, Manuel Scimeca, Julia Bischof, Jonathan Woodsmith, Massimiliano Agostini, Cristina Fiorani, Yufang Shi, Eleonora Candi, Gerry Melino, Alessandro Mauriello, Giuseppe S. Sica   +10 more
wiley   +1 more source

Differential Expression of Placental Glucocorticoid Receptors and Growth Arrest-Specific Transcript 5 in Term and Preterm Pregnancies: Evidence for Involvement of Maternal Stress

Obstetrics and Gynecology International, 2014
Pregnancy-specific stress predicts birth outcomes. We hypothesized that there is a maternal stress-GR interaction that can influence fetal birth weight.
D. Mparmpakas, E. Zachariades, G. Sotiriadis, A. Goumenou, A. J. Harvey, Y. Gidron, E. Karteris   +6 more
doaj   +1 more source