Results 231 to 240 of about 453,665 (301)

Evaluation of gene editing in CHO cells using the Cas‐CLOVER system

Biotechnology Progress, EarlyView.
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, Daniel Machado, Victor Cairns, Christine DeMaria, John J. Scarcelli   +13 more
wiley   +1 more source

Re-biopsy and modified biopsy in mosaic human embryos with simple segmental chromosomal deletion. [PDF]

Reprod Biol Endocrinol
Wang W, Liu B, Jin Z, Chen F, Wang X, Yin X, Shang W, Chuai Y.   +7 more
europepmc   +1 more source

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

The X Factor in Immunity: Sex Differences Shaped by the X Chromosome. [PDF]

Immunol Rev
Radovanovic KB, Wynalda M, Anguera MC, Anguera MC.   +3 more
europepmc   +1 more source

Making Two out of One: Kinesin Motors Driving Plant Cell Division

Cytoskeleton, EarlyView.
ABSTRACT The Kinesin superfamily of microtubule dependent motors is present in all eukaryotes. Not all of the subfamilies are represented in all kingdoms, and the ones that are do not always show conserved functions. Tight control of the cytoskeleton is essential for proper progression and completion of mitosis and cytokinesis, and key functions are ...
Choy Kriechbaum, Sabine Müller
wiley   +1 more source

Structural variations in evolutionary novel genomic regions: new insights into neurodevelopmental disorders by long-read DNA Sequencing. [PDF]

Mol Med
Rincic M, Kopic J, Klein V, Krsnik Z, Liehr T, Giesselmann S, Kurth I, Kraft F.   +7 more
europepmc   +1 more source

Discovery of an Atypical Arp2/3 Complex in Malaria Parasites Sheds New Light on Nuclear Actin

Cytoskeleton, EarlyView.
ABSTRACT The Arp2/3 complex is a key actin nucleator essential for cytoskeletal dynamics in eukaryotes. Previously believed absent in apicomplexan parasites, we recently identified an atypical Arp2/3 complex in malaria parasites consisting of five divergent subunits and a putative kinetochore‐associated factor.
Franziska Hentzschel, Friedrich Frischknecht, Matthias Marti   +2 more
wiley   +1 more source

Aneuploidy of chromosome 8 promotes the mesenchymal lineage during cell fate transitions. [PDF]

Sci Adv
Liang C, Li G, Zhang Q, Wang X, Liu Y, Yin W, Tao T, Wang J, Gao H, Qi S, Yu H.   +10 more
europepmc   +1 more source

Pharmacogenomics of Major Depressive Disorder in Indigenous Amazonian Populations

Clinical Pharmacology &Therapeutics, EarlyView.
Major depressive disorder is a highly prevalent psychological disorder worldwide and its main treatment is the use of Selective Serotonin Reuptake Inhibitors. However, few studies have demonstrated the relationship between the presence of genetic variants in pharmacogenes and the efficacy of these drugs, especially in populations with a unique genetic ...
Kaio Evandro Cardoso Aguiar, Natasha Monte da Silva, Juliana Carla Gomes Rodrigues, André Maurício Ribeiro‐dos‐Santos, Sandro José de Souza, Ândrea Ribeiro‐dos‐Santos, João Farias Guerreiro, Sidney Emanuel Batista dos Santos, Marianne Rodrigues Fernandes, Ney Pereira Carneiro dos Santos   +9 more
wiley   +1 more source