Results 91 to 100 of about 405,684 (326)

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster

Nature Communications
Modification of guanosine to N7-methylguanosine (m7G) in the variable loop region of tRNA is catalyzed by the METTL1/WDR4 heterodimer and stabilizes target tRNA. Here, we reveal essential functions of Mettl1 in Drosophila fertility.
Shunya Kaneko, Keita Miyoshi, Kotaro Tomuro, Makoto Terauchi, Ryoya Tanaka, Shu Kondo, Naoki Tani, Kei-Ichiro Ishiguro, Atsushi Toyoda, Azusa Kamikouchi, Hideki Noguchi, Shintaro Iwasaki, Kuniaki Saito   +12 more
doaj   +1 more source

Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17). [PDF]

PLoS ONE, 2013
In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity.
Hervé Acloque, Amélie Bonnet-Garnier, Florence Mompart, Alain Pinton, Martine Yerle-Bouissou   +4 more
doaj   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells

Nature Communications
Mitotic DNA synthesis (MiDAS) serves to complete the replication of genomic loci that are not fully replicated in S phase in response to replication stress.
Szymon A. Barwacz, Katrine Lundgaard, Wei Wu, Philipp H. Richter, Liqun Ren, Rahul Bhowmick, Marisa M. Gonçalves Dinis, Masato T. Kanemaki, Ying Liu   +8 more
doaj   +1 more source

Cytosolic Crowding Drives the Dynamics of Both Genome and Cytosol in Escherichia coli Challenged with Sub-lethal Antibiotic Treatments

iScience, 2020
Summary: In contrast to their molecular mode of action, the system-level effect of antibiotics on cells is only beginning to be quantified. Molecular crowding is expected to be a relevant global regulator, which we explore here through the dynamic ...
Michal Wlodarski, Leonardo Mancini, Bianca Raciti, Bianca Sclavi, Marco Cosentino Lagomarsino, Pietro Cicuta   +5 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Inferring chromosome radial organization from Hi-C data

BMC Bioinformatics, 2020
Background The nonrandom radial organization of eukaryotic chromosome territories (CTs) inside the nucleus plays an important role in nuclear functional compartmentalization. Increasingly, chromosome conformation capture (Hi-C) based approaches are being
Priyojit Das, Tongye Shen, Rachel Patton McCord   +2 more
doaj   +1 more source

Effects of human sex chromosome dosage on spatial chromosome organization. [PDF]

Mol Biol Cell, 2018
AbstractSex chromosome aneuploidies (SCAs) are common genetic syndromes characterized by the presence of an aberrant number of X and Y chromosomes due to meiotic defects. These conditions impact structure and function of diverse tissues, but the proximal effects of SCA on genome organization are unknown.
Jowhar Z, Shachar S, Gudla PR, Wangsa D, Torres E, Russ JL, Pegoraro G, Ried T, Raznahan A, Misteli T.   +9 more
europepmc   +3 more sources

Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]

, 1988
A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
B Trask, C Cremer, D Pinkel, D Pinkel, D. C. Ward, DA Agard, DJ Brigati, DJ Finney, GA Rappold, GD Johnson, GJ Brakenhoff, H Hamada, HE McDermid, HJ Cooke, J. Borden, JE Landegent, JG White, JR Gosden, JS Waye, L Hens, L Manuelidis, L Manuelidis, L Manuelidis, L Manuelidis, L Manuelidis, L. Manuelidis, LG Davis, M Schardin, MA Dilla Van, ML Mendelsohn, P. Lichter, PG Sealey, PR Langer, RJ Britten, T Cremer, T Cremer, T Cremer, T Cremer, T Maniatis, T. Cremer   +39 more
core   +1 more source