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Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding
2023Recently developed bulked oligo-FISH is a highly versatile method, which is applicable in any plant species with an assembled genome sequence. This technique allows in situ identification of individual chromosomes, large chromosomal rearrangements, comparative karyotype analysis, or even the reconstruction of the three-dimensional organization of the ...
Denisa, Beránková, Eva, Hřibová
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Chromosome painting — principles, strategies and scope
Methods in Cell Science, 2001Chromosome Painting is emerging as a powerful tool in the exact localization of different gene sequences of chromosomes at the microscopic level. It is principally based on molecular hybridization in situ with sequence specific probes on chromosomes. Different strategies have been adopted for the preparation of probes, hybridization and visualization ...
A K, Sharma, A, Sharma
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Analysis of Plant Meiotic Chromosomes by Chromosome Painting
2013Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP)
Martin A, Lysak, Terezie, Mandáková
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Cytogenetic analysis by chromosome painting
Cytometry, 1994AbstractChromosome painting is a term used to describe the direct visualisation using in situ hybridisation of specific chromosomes in metaphase spreads and in interphase nuclei. Chromosome painting, coupled with fluorescence in situ hybridisation (FISH), is now used routinely to enhance the identification of chromosomal rearrangements, the assignment ...
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Comparative chromosome painting
2000Chromosome paint probes are complex genomic probes prepared by random PCR amplification of chromosome samples. Most chromosome samples are obtained by sorting a fluid suspension of chromosomes in a dual laser fluorescence-activated cell sorter [1] although microdissection of chromosomes or chromosome regions may also be used [2].
Malcolm A. Ferguson-Smith +3 more
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Phylogenomic Analysis by Chromosome Sorting and Painting
2008Chromosome sorting by flow cytometry is the principle source of chromosome-specific DNA not only for chromosome painting, but also for many other types of genomic analysis such as library construction, discovery and isolation of genes, chromosome specific direct DNA selection, and array painting. Chromosome sorting coupled with chromosome painting is a
STANYON, ROSCOE ROBERT, G. Stone
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Chromosome painting of radiation-induced micronuclei
International Journal of Radiation Biology, 1996In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of the numbers of signal-positive MN ...
A, Slavotinek +4 more
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Chromosome painting in acute monocytic leukemia
Genes, Chromosomes and Cancer, 1993AbstractAn Alu polymerase chain reaction (PCR) probe specific for chromosome II prepared from the somatic cell hybrid JI was used to analyze karyotypes of eight patients with acute monocytic leukemia (AML‐M5). Chromosome painting confirmed the t(9;II) in one patient and a der(I)t(I;6)t(6;II) in another and allowed the identification of a complex ...
D, Cherif +4 more
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A procedure for image enhancement in chromosome painting
Chromosome Research, 2006An image enhancement procedure was developed to produce high-contrast chromosome paint images. This procedure is well suited for images where brightness-contrast enhancement is subjective. Three examples are given to show that the procedure is very efficient to remove non-specific hybridization signals from the chromosome paint image.
W, Rens +5 more
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1997
Chromosome painting is a technique that allows the specific visualization of an entire chromosome in metaphase spreads and in interphase nuclei by in situ hybridization with a mixture of sequences generated from that particular chromosome (Pinkel et al., 1988; Carter, 1994).
J. M. Vega +3 more
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Chromosome painting is a technique that allows the specific visualization of an entire chromosome in metaphase spreads and in interphase nuclei by in situ hybridization with a mixture of sequences generated from that particular chromosome (Pinkel et al., 1988; Carter, 1994).
J. M. Vega +3 more
openaire +1 more source