Results 1 to 10 of about 133,106 (256)

Interphase fluorescence in situ hybridization assisting in prenatal counseling for amniocentesis karyotyping-detected fetal mosaicism

open access: yesTaiwanese Journal of Obstetrics & Gynecology, 2015
Objective: To evaluate how interphase fluorescence in situ hybridization (FISH) played a role in genetic counseling when encountering prenatally detected fetal mosaicism cases. Materials and methods: We retrospectively reviewed 17 cases of amniotic fluid
Sheng-Yuan Su   +5 more
doaj   +1 more source

Testing the Potential Clastogenic/Cytotoxic Effects of Pesticide CALYPSO 480 SC

open access: yesFolia Veterinaria, 2017
The detection of chromosomal damage serves as a tool for the verification of the genotoxic effects of chemical substances in vitro. We used conventional cytogenetic analysis in order to test for the potential genotoxic action of the insecticide ...
Ficová I., Galdíková M.
doaj   +1 more source

Distinction Between Paternal and Maternal Contributions to the Tripronucleus in Human Zygotes Obtained after In Vitro Fertilization

open access: yesTaiwanese Journal of Obstetrics & Gynecology, 2006
Objective: The aim of this study was to determine the genetic composition of tripronuclear (3PN) zygotes. Fluorescence in situ hybridization (FISH) was used to quantify the sperm tails in human 3PN zygotes. Materials and Methods: Ovarian hyperstimulation
San-Li Yu   +6 more
doaj   +1 more source

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

open access: yesActa Medica Bulgarica, 2017
Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was
Kotzev A., Kamenova M.
doaj   +1 more source

A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection

open access: yesScience and Technology of Advanced Materials, 2016
We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target,
Hiromu Kashida   +2 more
doaj   +1 more source

Fluorescent in situ hybridization has limitations in screening NRG1 gene rearrangements

open access: yesDiagnostic Pathology
Background NRG1 fusion is a promising therapeutic target for various tumors but its prevalence is extremely low, and there are no standardized testing algorithms for genetic assessment. Mothods In this study, we analyzed 3008 tumors using Fluorescence in
Xiaomei Zhang   +9 more
doaj   +1 more source

Swollen lymph nodes may not be clinical manifestations of chronic myeloid leukemia: case report and revision of literature

open access: yesTherapeutics and Clinical Risk Management, 2017
Zhihe Liu,1 Siyun Li,2 Ou Bai1 1Department of Hematology, The First Hospital of Jilin University, Changchun, 2Department of Pediatrics, Women and Children’s hospital of Qingdao university, Qingdao, People’s Republic of China Abstract: We ...
Liu Z, Li S, Bai O
doaj  

Fluorescence In Situ Hybridization in Whole-Mount Drosophila Embryos

open access: yesBioTechniques, 1996
Sarah C. Hughes   +3 more
doaj   +1 more source

VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES

open access: yesВавиловский журнал генетики и селекции, 2014
Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed,
A. G. Bogomolov   +4 more
doaj  

Fluorescence In Situ Hybridization

open access: yes
FISH and the related in situ hybridization (ISH) techniques provide complementary genomic information to qPCR, ddPCR, NGS, and NanoString techniques. This technology was initially developed as a physical mapping tool to delineate genes within chromosomes. Its high analytical resolution to a single gene level and high sensitivity and specificity enables
openaire   +1 more source

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