Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
, 1994 We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Kilian, Karin +6 more
core +1 more source
Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization
Frontiers in Molecular Neuroscience, 2022 Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka +8 more
doaj +1 more source
Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution [PDF]
, 2005 For the last 15 years molecular cytogenetic techniques have been extensively used to study primate evolution. Molecular probes were helpful to distinguish mammalian chromosomes and chromosome segments on the basis of their DNA content rather than solely ...
Wienberg, Johannes, Wienberg, J.
core +1 more source
Haematologica, 2008 Background More than 50% of patients with myelodysplastic syndromes present cytogenetic aberrations at diagnosis. Partial or complete deletion of the long arm of chromosome 5 is the most frequent abnormality.
Mar Mallo +41 more
doaj +1 more source
Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]
, 1990 A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Stanyon, Roscoe +3 more
core +1 more source
Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]
, 1990 Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Thomas +4 more
core +1 more source
Fluorescent in Situ Hybridization [PDF]
, 2008 In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
openaire +2 more sources
A simplified and rapid in situ hybridization protocol for planarians
BioTechniques, 2023 Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj +1 more source
Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization [PDF]
, 1993 Comparative genomic in situ hybridization (CGH) provides a new possibility for searching genomes for imbalanced genetic material. Labeled genomic test DNA, prepared from clinical or tumor specimens, is mixed with differently labeled control DNA prepared ...
Speicher, Michael R. +9 more
core +1 more source
A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
, 2009 Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Müller, Stefan +4 more
core +1 more source