Results 311 to 320 of about 840,795 (338)
Some of the next articles are maybe not open access.
Microdissection and chromosome painting of plant B chromosomes
2001Plant chromosome microdissection techniques together with different isolation and amplification methods of microisolated DNA are described. Such isolated DNA was used to 'chromosome paint' B chromosomes of the dicot Brachycome dichromosomatica and the monocot Secale cereale. It is demonstrated that the specific painting of the described chromosomes was
Andreas Houen +2 more
openaire +3 more sources
Chromosome ?painting? in plants ? a feasible technique?
Chromosoma, 1996It is shown that chromosome painting is as yet not possible for plants with very complex genomes, neither intra- nor interspecific. The reasons are inefficient blocking of dispersed repetitive sequences and insufficient signal intensity of short unique sequences. Future perspective are indicated.
Andreas Houben +3 more
openaire +3 more sources
Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding
2023Recently developed bulked oligo-FISH is a highly versatile method, which is applicable in any plant species with an assembled genome sequence. This technique allows in situ identification of individual chromosomes, large chromosomal rearrangements, comparative karyotype analysis, or even the reconstruction of the three-dimensional organization of the ...
Denisa, Beránková, Eva, Hřibová
openaire +2 more sources
Phylogenomic Analysis by Chromosome Sorting and Painting
2008Chromosome sorting by flow cytometry is the principle source of chromosome-specific DNA not only for chromosome painting, but also for many other types of genomic analysis such as library construction, discovery and isolation of genes, chromosome specific direct DNA selection, and array painting. Chromosome sorting coupled with chromosome painting is a
STANYON, ROSCOE ROBERT, G. Stone
openaire +4 more sources
Chromosome Painting Using Chromosome-Specific BAC Clones
2023Chromosome painting (CP) refers to visualization of large chromosome regions, chromosome arms or entire chromosomes via fluorescence in situ hybridization (FISH) of chromosome-specific DNA sequences. For CP in crucifers (Brassicaceae), typically contigs of chromosome-specific bacterial artificial chromosomes (BAC) from Arabidopsis thaliana are applied ...
Terezie M, Mandáková, Martin A, Lysak
openaire +2 more sources
Chromosome painting in acute monocytic leukemia
Genes, Chromosomes and Cancer, 1993AbstractAn Alu polymerase chain reaction (PCR) probe specific for chromosome II prepared from the somatic cell hybrid JI was used to analyze karyotypes of eight patients with acute monocytic leukemia (AML‐M5). Chromosome painting confirmed the t(9;II) in one patient and a der(I)t(I;6)t(6;II) in another and allowed the identification of a complex ...
Dorra Cherif +5 more
openaire +2 more sources
Chromosome painting of radiation-induced micronuclei
International Journal of Radiation Biology, 1996In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of the numbers of signal-positive MN ...
S Sauer-Nehls +4 more
openaire +3 more sources
Identification of bacterial cells by chromosomal painting
Applied and Environmental Microbiology, 1997Chromosomal painting is a technique for the microscopic localization of genetic material. It has been applied at the subcellular level to identify regions of eukaryotic chromosomes. Here we describe the development of bacterial chromosomal painting (BCP), a related technology for the identification of bacterial cells.
Stephen J. Giovannoni, Brian Lanoil
openaire +3 more sources
A procedure for image enhancement in chromosome painting
Chromosome Research, 2006An image enhancement procedure was developed to produce high-contrast chromosome paint images. This procedure is well suited for images where brightness-contrast enhancement is subjective. Three examples are given to show that the procedure is very efficient to remove non-specific hybridization signals from the chromosome paint image.
Malcolm A. Ferguson-Smith +5 more
openaire +3 more sources
A new chromosome was born: comparative chromosome painting in Boechera
Trends in Plant Science, 2015Comparative chromosome painting is a powerful tool to study the evolution of chromosomes and genomes. Analyzing karyotype evolution in cruciferous plants highlights the origin of aberrant chromosomes in apomictic Boechera and further establishes the cruciferous plants as important model system for our understanding of plant chromosome and genome ...
openaire +3 more sources