Differences in the localization and morphology of chromosomes in the human nucleus [PDF]
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19.
Perry, P +5 more
core +7 more sources
Sorting of chromosomes by magnetic separation [PDF]
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
Dudin, Gertrud +7 more
core +1 more source
Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes [PDF]
Copyright: © 2010 Stimpson et al.Genome rearrangement often produces chromosomes with two centromeres (dicentrics) that are inherently unstable because of bridge formation and breakage during cell division. However, mammalian dicentrics, and particularly
Sullivan, Beth A. +35 more
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Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes [PDF]
Studies of higher-order chromatin arrangements are an essential part of ongoing attempts to explore changes in epigenome structure and their functional implications during development and cell differentiation.
Kreth Gregor +54 more
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Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Speicher, Michael R. +5 more
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Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers [PDF]
Specific amplification of human sequences of up to several kb length has recently been accomplished in man-hamster and man-mouse somatic hybrid cell DNA by IRS-PCR (interspersed repetitive sequence — polymerase chain reaction).
Riethman, Harold C. +2 more
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Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region [PDF]
The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid.
Houben, A. +15 more
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Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates interphase chromosome territories [PDF]
In spite of Carl Rabl's (1885) and Theodor Boveri's (1909) early hypothesis that chromosomes occupy discrete territories or domains within the interphase nucleus, evidence in favor pf this hypothesis has been limited and indirect so far in higher plants ...
Schardin, Margit +3 more
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Independent stratum formation on the avian sex chromosomes reveals inter-chromosomal gene conversion and predominance of purifying selection on the w chromosome [PDF]
We used a comparative approach spanning three species and 90 million years to study the evolutionary history of the avian sex chromosomes. Using whole transcriptomes, we assembled the largest cross-species dataset of W-linked coding content to date.
Wright, Alison E +9 more
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Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution [PDF]
For the last 15 years molecular cytogenetic techniques have been extensively used to study primate evolution. Molecular probes were helpful to distinguish mammalian chromosomes and chromosome segments on the basis of their DNA content rather than solely ...
Wienberg, Johannes, Wienberg, J.
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