, 2011 We present a novel and efficient non-integrating gene expression system in human embryonic stem cells (hESc) utilizing human artificial chromosomes (HAC), which behave as autonomous endogenous host chromosomes and segregate correctly during cell division.
Moralli, D. +36 more
core +1 more source
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]
, 1988 A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
Cremer, Thomas +4 more
core +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
FEBS Open Bio, EarlyView.This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
CENP-B: Cornerstone of Kinetochores in Centromeres of Human Chromosomes
Frontiers in Bioscience-LandmarkEach chromosome contains a centromere, the site at which the kinetochore assembles to ensure accurate chromosome segregation during cell division. Centromeric chromatin, which anchors the kinetochore, includes three core proteins: Centromere Protein A ...
Fritz F. Parl
doaj +1 more source
The Effect of Teratozoospermia on Sex Chromosomes in Human Embryos
The Application of Clinical Genetics, 2021 Dalia Mostafa Nayel,1 Hanan Salah El Din Mahrous,1 Emad El Din Khalifa,2 Soha Kholeif,1 Ghada Mohamed Elhady1 1Department of Human Genetics, Medical Research Institute, Alexandria University, Alexandria, Egypt; 2Department of Obstetrics and Gynecology ...
Mostafa Nayel D +4 more
doaj
c-Myc—Dependent Formation of Robertsonian Translocation Chromosomes in Mouse Cells
Neoplasia: An International Journal for Oncology Research, 2007 Robertsonian (Rb) translocation chromosomes occur in human and murine cancers and involve the aberrant joining of two acrocentric chromosomes in humans and two telocentric chromosomes in mice.
Amanda Guffei +5 more
doaj +1 more source
Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]
, 1992 Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
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, 1967 Sex Chromosomes focuses on the study of sex chromosomes, including human chromosomal abnormalities, behavior and characteristics of chromosomes, and cell division.
Mittwoch, Ursula
core
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source