Results 61 to 70 of about 15,767 (185)

Emerging role of metagenomic next‐generation sequencing in infectious disease diagnostics: Clinical integration and future directions

mLife, Volume 5, Issue 2, Page 148-163, April 2026.
Abstract Infectious disease diagnostics has been transformed by metagenomic next‐generation sequencing (mNGS), an unbiased approach that detects bacteria, viruses, fungi, and parasites in a single assay. By sequencing all nucleic acids in a sample, mNGS overcomes the narrow detection scope and slow turnaround of conventional tests, substantially ...
Tingting Fang, Feifei Yuan, Yu Chen, Na Li, Yao Zhang, Haixia Liu, Xingchen Liu, Qing Miao, Bijie Hu   +8 more
wiley   +1 more source

Meta-Transcriptomic Discovery of a Divergent Circovirus and a Chaphamaparvovirus in Captive Reptiles with Proliferative Respiratory Syndrome

Viruses, 2020
Viral pathogens are being increasingly described in association with mass morbidity and mortality events in reptiles. However, our knowledge of reptile viruses remains limited. Herein, we describe the meta-transcriptomic investigation of a mass morbidity
Wei-Shan Chang, Ci-Xiu Li, Jane Hall, John-Sebastian Eden, Timothy H. Hyndman, Edward C. Holmes, Karrie Rose   +6 more
doaj   +1 more source

Development of a Novel Polyleucine‐Based Immunopotentiator for Subunit Vaccines Against Group A Streptococcus

Small, Volume 22, Issue 22, 17 April 2026.
Self‐assembling amphiphilic peptide L15K6 is used as a novel immunoadjuvant for the development of a Group A Streptococcus vaccine. Robust antigen‐specific IgG responses are generated and manage to kill isolated strains of Group A Streptococcus. Cellular and bulk RNA sequencing data reveals that L15K6 regulates immune responses potentially through TLR ...
Lantian Lu, Jazmina L. Gonzalez Cruz, Rabina Giri, Yuexiao Zhang, Sahra Bashiri, Leslie C. Domínguez Cadena, Shuhang Li, Zeinab G. Khalil, Waleed M. Hussein, Jingwen Wang, Jolynn Kiong, Xuehan Lu, Jiahui Zhang, Miao Lu, Wenbin Huang, Wei Yang Kong, Ummey Jannatun Nahar, Prashamsa Koirala, Zhong Guo, Patricia Walden, Antje Blumenthal, Jakob Begun, Kirill Alexandrov, Istvan Toth, Rachel J. Stephenson, Mariusz Skwarczynski   +25 more
wiley   +1 more source

The detection of canine circovirus in Guangxi, China

Virus Research, 2019
Since the first description of canine circovirus (CanineCV)-associated infection, there have been several reports on the distribution of the disease in worldwide. To investigate the prevalence and genetic diversity of CanineCV in China, we conducted PCR screening of 1226 dog serum samples collected from different regions in mainland China between 2014 ...
Wenchao Sun, Hongyun Zhang, Min Zheng, Huihui Cao, Huijun Lu, Guanyu Zhao, Changzhan Xie, Liang Cao, Xiankai Wei, Jingshan Bi, Chizhe Yi, Gefen Yin, Ningyi Jin   +12 more
openaire   +2 more sources

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source

Potential New Avian Species as Carriers of Diverse Circoviruses

Pathogens
Avian species pose risks for transmitting viruses, including avian circoviruses, that could be a threat for conservation and introduction into commercial flocks.
Yasmin Luisa Neves Lemes Garcia, Ana Júlia Chaves Gomes, Guilherme Guerra Neto, Natasha Fujii Ando, Camila Sanches Rodrigues, Richard Alegria Cesario, Camila Domit, Fábio Henrique Lima, Helena Lage Ferreira, João Pessoa Araújo, Bruna Lindolfo da Silva, Fernando Rosado Spilki, Luciano Matsumiya Thomazelli, Thais Helena Martins Gamon, Isabela Barbosa Assis, Edison Luiz Durigon, Danielle Bruna Leal Oliveira, Vivaldo Gomes da Costa, Marília de Freitas Calmon, Paula Rahal   +19 more
doaj   +1 more source

Safeguarding a Flagship Species: Integrated Surveillance of Cross‐Species Pathogen Transmission in Giant Panda Ecosystems

Ecology and Evolution, Volume 16, Issue 3, March 2026.
Emerging infectious diseases increasingly threaten giant pandas and cohabiting species. This review analyzes pathogen transmission risks from domestic animals, wildlife, and vectors and proposes an integrated genomic surveillance framework for early warning and biodiversity conservation.
Xiaoli Sun, Yi Peng, Xiaoye Hao, Rong Dong, Zhilin Wang, Le Wang, Chengdong Wang, Xiangdong Wu, Zheng Chen, Wenbo Zhang, Xiaoli Tang   +10 more
wiley   +1 more source

Application of Quantitative PCR (qPCR) for the Detection of Psittacine Beak and Feather Disease Virus (PBFDV) in Air Conditioning Systems: A Model Study from a Veterinary Hospital in Thailand

Veterinary Sciences
Psittacine beak and feather disease virus (PBFDV), a member of the family Circoviridae, is a major pathogen affecting psittacine birds worldwide; however, its potential for airborne dissemination in veterinary environments remains poorly understood. This
Benchapol Lorsunyaluck, Juthanan Charachit, Sasipat Putsetkun, Natapol Pumipuntu   +3 more
doaj   +1 more source

A novel kirkovirus may be associated with equine gastrointestinal disease

Equine Veterinary Journal, Volume 58, Issue 2, Page 414-422, March 2026.
Abstract Background Many cases of equine enterocolitis are suspected to be infectious in nature, but no pathogen is identified in many cases. Objectives Perform next‐generation sequencing on faeces collected from cases of equine enterocolitis for the presence of novel viruses and determine if an identified novel virus is associated with cases of equine
Lillian M. B. Haywood, Ava Clark, Ben Hause, Breanna Sheahan   +3 more
wiley   +1 more source

Pathogenesis and Transmission of a Reassorted H1 Influenza A Virus Detected in North American Swine

Influenza and Other Respiratory Viruses, Volume 20, Issue 2, February 2026.
ABSTRACT Background The USDA influenza A virus in swine national surveillance plan identified an increase in the detection frequency of a group of swine 1A.1.1.3 hemagglutinin (HA) clade viruses. This change was associated with reassortment events that resulted in new neuraminidase (NA) gene pairings.
Débora B. Goulart, Carine K. Souza, Giovana C. Zanella, Celeste A. Snyder, Janice C. Zanella, Alexey Markin, Bailey Arruda, Tavis K. Anderson, Amy L. Baker   +8 more
wiley   +1 more source