Results 71 to 80 of about 6,300 (231)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
An O(n1.5) algorithm to color proper circular arcs
Discrete Applied Mathematics, 1989 AbstractA circular-arc family F is a collection of arcs on a circle. Such a family is said to be proper if no arc is contained within another. The coloring problem on circular-arc families has been shown to be NP-hard by Garey, Johnson, Miller and Papadimitriou.
Wei-Kuan Shih, Wen-Lian Hsu
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FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
The Strawberry Quick Problem: A devil in disguise
Journal of Bahria University Medical and Dental College, 2017 To, The editor, I would like to report the recent Strawberry Quick drug scare which has now reached Pakistan, to add to its plethora of bewildering issues.
Hira Saleem +2 more
doaj
Winding number and circular coloring
In 1996, Youngs proved a surprising theorem that quadrangulations of the projective plane could never have chromatic number exactly 3. This sparked a lot of interest, and the result has been further developed in many directions over the past decades. For example, the result is strengthened by considering the circular chromatic number, which is a real ...
Naserasr, Reza +2 more
openaire +2 more sources
A connection between circular colorings and periodic schedules
Discrete Applied Mathematics, 2009 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
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RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source