Results 61 to 70 of about 208,265 (305)

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.

PLoS ONE, 2009
Extrachromosomal circular DNA (eccDNA) is a pool of circular double stranded DNA molecules found in all eukaryotic cells and composed of repeated chromosomal sequences. It was proposed to be involved in genomic instability, aging and alternative telomere
Zoya Cohen, Sara Lavi
doaj   +1 more source

Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Nature Protocols, 2020
Circular DNA is ubiquitous in nature in the form of plasmids, circular DNA viruses, and extrachromosomal circular DNA (eccDNA) in eukaryotes. Sequencing of such molecules is essential to profiling virus distributions, discovering new viruses and understanding the roles of eccDNAs in eukaryotic cells.
Devang Mehta, Luc Cornet, Matthias Hirsch-Hoffmann, Syed Shan-e-Ali Zaidi, Hervé Vanderschuren   +4 more
openaire   +5 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

TempliPhi, ⌽29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing

BioTechniques, 2002
We have developed a novel, isothermal DNA amplification strategy that employs ⌽29 DNA polymerase and rolling circle amplification to generate high-quality templates for DNA sequencing reactions. The TempliPhi™ DNA amplification kits take advantage of the
John R. Nelson, Yuyang Christine Cai, Theresa L. Giesler, Joseph W. Farchaus, Shanmuuga T. Sundaram, Maria Ortiz-Rivera, Lou P. Hosta, Peter L. Hewitt, J. Anthony Mamone, C. Palaniappan, Carl W. Fuller   +10 more
doaj   +1 more source

The role of extra chromosomal circular DNA in rapid adaption to glyphosate resistance in pigweed [PDF]

, 2021
W Molin, E Patterson, C Saski, W Molin, A Yaguchi, M Blenner, C Saski, W Molin, A Yaguchi, M Blenner, C Saski, Dal-Hoe Koo, W Molin, A Wright, A Lawton-Rauh, C Saski   +15 more
openalex   +1 more source

Denaturation of circular DNA: Supercoil mechanism

Physical Review E, 2011
The denaturation transition which takes place in circular DNA is analyzed by extending the Poland-Scheraga model to include the winding degrees of freedom. We consider the case of a homopolymer whereby the winding number of the double stranded helix, released by a loop denaturation, is absorbed by \emph{supercoils}.
Bar, Amir, Kabakcioglu, Alkan, Mukamel, David   +2 more
openaire   +5 more sources

Mechanisms of parasite‐mediated disruption of brain vessels

FEBS Letters, EarlyView.
Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira, Sílvia Arroz‐Madeira, Cláudio A. Franco, Sara Silva Pereira   +3 more
wiley   +1 more source

Entropic penalties in circular DNA assembly [PDF]

The Journal of Chemical Physics, 2014
The thermodynamic properties of DNA circular molecules are investigated by a new path integral computational method which treats in the real space the fundamental forces stabilizing the molecule. The base pair and stacking contributions to the classical action are evaluated separately by simulating a broad ensemble of twisted conformations.
openaire   +4 more sources