Results 101 to 110 of about 60,465 (252)

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong, Dingyi Yu, Nan Hao, Chris Langendorf, Keith. E. Shearwin, Jessica K. Holien   +5 more
wiley   +1 more source

Spray‐Dried Engineered Escherichia coli Whole‐Cell Biocatalysts Enable Cell Recycling and In Vivo α‐Ketoglutarate and NADPH Regeneration for Efficient Codeine Manufacture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A recombinant whole‐cell biocatalytic approach provides several advantages over isolated enzymes, such as improved enzyme stability, in vivo cofactor regeneration, and the facilitation of cascade reactions within a single cell. Whole‐cell biocatalysis has gained attention in medicinal opioid production, due to its technical and economic ...
Ali Jahanian, Carlos Horacio Luna‐Flores, Xu Li, Sally L. Gras, Robert E. Speight   +4 more
wiley   +1 more source

Targeting neutrophil extracellular traps in metabolic and immune niche: Nanomaterials for diabetes tissue regeneration

BMEMat, EarlyView.
The effects of NETs on regeneration of various diabetic tissues, and strategies targeting NETs for diabetes tissue regeneration. In the diabetic environment, NETs undergo complex metabolic and immune reprogramming, leading to dynamic changes in antibacterial and proinflammatory functions, and affecting regeneration of multiple systemic tissues.
Xinyi Jiang, Muxin Yue, Kai Mao, Boon Chin Heng, Noor Azlin Yahya, Yunsong Liu, Zheng Li   +6 more
wiley   +1 more source

Multifunctional hyaluronic acid hydrogel integrating exosome‐encapsulating gelatin microspheres accelerates skeletal muscle regeneration via TGF‐β1 activation and enhanced myogenic cell viability

BMEMat, EarlyView.
Schematic illustration of preparation of injectable H/G@Co@miR‐206 hydrogel for efficient skeletal muscle regeneration in cardiotoxin‐induced skeletal muscle injury SD rat model. Abstract The effective treatment of acute skeletal muscle injuries remains challenging, necessitating therapeutic strategies that concurrently reestablish vascular perfusion ...
Zhengzhe Han, Zongyue Li, Ang Li, Zheng Jiang, Saiyu Shi, Zhipeng Wang, Jishizhan Chen, Yun Qian, Yuanyang Gu, Xianyou Zheng   +9 more
wiley   +1 more source

Association of plasma metabolites and cardiac mitochondrial function with heart failure progression

ESC Heart Failure
Aims Plasma metabolites are prognostic in heart failure with reduced ejection fraction (HFrEF), with citric acid cycle metabolites linked to ejection fraction (EF) changes. We investigated these mechanisms in a canine chronic HFrEF model.
Vandana Revathi Venkateswaran, Ruicong She, Stephen J. Gardell, Jasmine A. Luzum, Ramesh Gupta, Kefei Zhang, L. Keoki Williams, Hani N. Sabbah, David E. Lanfear   +8 more
doaj   +1 more source

Sequential catalytic nanosystem of carbon dots and uricase integrated with polydopamine for therapy of gouty wound ulceration

BMEMat, EarlyView.
Sequential dual‐catalytic treatment of the gout wound ulceration process by CDs‐Uri@PDA. (a) Synthesis steps and morphology of CDs‐Uri@PDA. (b) Sequential treatment process and mechanism of CDs‐Uri@PDA for gout wound ulceration according to (i) uric acid reduction, (ii) antibacterial activity, and (iii) anti‐inflammatory effects.
Yonglan Yang, Yang Yang, Pengpeng Ma, Ruiqi Zhu, Xia Pan, Shaoling Zheng, Bo Chen, Yanli Jin, Youcong Gong, Jie Liu, Tianwang Li, Xu Chen   +11 more
wiley   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Protein hydrolysates in cell culture: Toward multi‐omics characterization

Biotechnology Progress, EarlyView.
While protein hydrolysates are widely used in cell culture applications, they remain undefined and variable products. Multi‐omic characterization evaluating composition and function can transition hydrolysates toward semi‐defined media components.
Michelle Combe, Brandon M. Wrage, Angel Varela‐Rohena, Stanislav Sokolenko   +3 more
wiley   +1 more source

Carbon Quantum Dots Meet Perovskite Solar Cells: A Review of Multifunctional Synergies

Carbon Energy, EarlyView.
This review highlights the multifunctional role of carbon dots in perovskite solar cells. Their applications as additives and interfacial modifiers are discussed in terms of crystallization control, defect passivation, energy‐level alignment, and environmental stability.
Yagmur Su Sayin, Matteo Bonomo, Nilgun Karatepe, Marta Feroci   +3 more
wiley   +1 more source

High Entropy–Driven Performance Enhancement in Perovskite Oxides for Energy Conversion and Storage Systems

Carbon Energy, EarlyView.
High‐entropy perovskite oxides enable tunable structures and properties through multi‐cation disorder and entropy stabilization. This review summarizes advances in synthesis, structural and defect engineering, and their applications in OER/ORR, CO2RR, and electrochemical energy storage, highlighting in situ characterization and modeling that clarify ...
Obeylaw Moyo, Jiaqiao Yang, Mingzhuang Liang, Hyunseung Kim, Wei Wang, Yong Beom Kim, Junxiong Zhang, WooChul Jung, Hainan Sun   +8 more
wiley   +1 more source