Results 221 to 230 of about 696,030 (310)

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Secretory immunoglobulin A (sIgA) is a promising emerging biopharmaceutical candidate, but it currently lacks a standardized platform for purification. To address this, a novel affinity chromatography resin was developed by immobilizing protein variants of the Streptococcus pneumoniae surface protein, SpsA, as an affinity ligand.
David Scheich, Andres Männik, Oliver Meikar, Joan Teyra, Laura Kibena, Sebastiaan Eeltink, Rainer Hahn, Alois Jungbauer, Nico Lingg   +8 more
wiley   +1 more source

Enhancing the Durability of Bituminous Concrete Using Plastic Waste on Soft Rock Aggregates. [PDF]

Polymers (Basel)
Laldintluanga H, Zorinkima, Ramhmachhuani R.   +2 more
europepmc   +1 more source

Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy

Biotechnology and Bioengineering, EarlyView.
Schematic depicting the workflow for model building and validation using Raman spectroscopy for real‐time monitoring of IVT. ABSTRACT The success of COVID‐19 mRNA vaccines showcased the transformative potential of this adaptable, plug‐and‐play, and rapid response platform.
Julia Puppin Chaves Fulber, Xingge Xu, Hadi El Radi, Kevin Grollier, Amine A. Kamen   +4 more
wiley   +1 more source

Film and Diffusion Kinetics of Ammonium Removal from Leachate in an Up-Flow Anaerobic Sludge Blanket Reactor. [PDF]

ACS Omega
Fulazzaky MA, Hariati F, Susetyo B, Yuniarto A, Afrianto Y, Sofyan A, Yuzir A, Zulaika A, Muda K.   +8 more
europepmc   +1 more source

Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho, Tayyab Islam, Sobia Islam, Junhak Lee, Sung Won Jung, Sunghoon Park   +5 more
wiley   +1 more source

Impact of Process Interruptions in the Production of Lysates for Cell‐Free Expression Systems

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free gene expression systems offer cell‐like functionalities outside the confines of the cell, garnering increasing interest for applications from biomanufacturing to sensing. As applications expand, the need to implement economically scaled processes to produce cellular lysates grows.
Katherine A. Rhea, Ethan N. Walters, Julie L. Zacharko, Mia J. Seergae, Thomas R. Laws, Thomas C. Maishman, Yun‐Nam Choi, John T. Lazar, Ashty Karim, Weston Kightlinger, Michael C. Jewett, Matthew W. Lux   +11 more
wiley   +1 more source

The 2015 Nepal Gorkha Earthquake: An overview of the damage, lessons learned and challenges [PDF]

, 2016
Associate Professor, Department of Civil Engineering, Kyushu University, Fukuoka, JAPAN, Associate Professor, Department of Civil Engineering, Kyushu University, Fukuoka, JAPAN, Associate Professor, Graduate School of Science and Engineering, Ehime University, Matsuyama, JAPAN, Bhandary, N.P., Hazarika, H., Kajita, Y., Kasama, K., Pokharel, R.K., Professor, Department of Civil Engineering, Kyushu University, Fukuoka, JAPAN, Professor, Department of Urban and Environmental Engineering, Kyushu University, Fukuoka, JAPAN, Professor, Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka, JAPAN, Tsukahara, K.   +11 more
core  

Genetically Programmed Control of Overflow Metabolism

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen, Andriy Glushchuk, Thilde P. Hjorth, Alvaro R. Lara   +3 more
wiley   +1 more source

A Prediction Model of Interlayer Bond Strength for 3D-Printed Concrete Considering Printing Interval and Environmental Effects. [PDF]

Materials (Basel)
Xu W, Xu Z, Liu T, Ouyang J, Wang J, Wang H, Xu W.   +6 more
europepmc   +1 more source

Advancing Malic Acid Production of Aspergillus oryzae: Combined Process Optimization and Application of Various Substrates

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In light of climate change and growing resource scarcity, microbial production of organic acids offers a sustainable alternative to fossil‐based chemical synthesis. In this study, malic acid production by Aspergillus oryzae was optimized through cultivation temperature adjustment and biotin supplementation, while organic acid formation from ...
Lukas Hartmann, Anke Neumann, Dirk Holtmann, Björn Vater, Thomas Hahn, Susanne Zibek, Katrin Ochsenreither   +6 more
wiley   +1 more source