Results 181 to 190 of about 75,978 (312)

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Advancing Civil Engineering Education: Implications of Using Augmented Reality in Teaching Structural Analysis

As technological advances appear, it is desirable to integrate them into new engineering education teaching methods, aiming to enhance students' comprehension and engagement with complex subjects.
Jahren, Charles, Miner, Nathan, Alipour, Alice, Karabulut‐Ilgu, Aliye   +3 more
core  

Fouling‐Free Inertial Microfluidic Cell Clarification for Lentivirus Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Lentiviral vectors (LVVs) are widely used as gene‐delivery vehicles in cell and gene therapy, enabling stable integration of transgenes into target cells. However, efficient clarification of LVV harvest remains a major bottleneck in large‐scale manufacturing, as conventional depth filtration often results in significant product loss.
Dohyun Park, Alexander Bevacqua, Mingyang Cui, Marina Nichols, Jianzhu Chen, Jongyoon Han   +5 more
wiley   +1 more source

Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling

Biotechnology and Bioengineering, EarlyView.
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro, José Luis Checa‐Barrera, Juan A. Moreno‐Cid, Eoin Casey   +3 more
wiley   +1 more source

Author Correction: Kriging-based surrogate data-enriching artificial neural network prediction of strength and permeability of permeable cement-stabilized base. [PDF]

Nat Commun
Wang X, Xiao Y, Li W, Wang M, Zhou Y, Chen Y, Li Z.   +6 more
europepmc   +1 more source

Model‐Enabled Knowledge Transfer Across Cell Lines, Culture Scales and Conditions

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Mechanistic models are central to quantitative understanding and optimization of Chinese hamster ovary (CHO) cell culture processes, but their utility is often restricted by parameter sets calibrated for specific cell lines, scales, or operating conditions.
Luxi Yu, Antonio del Rio Chanona, Cleo Kontoravdi   +2 more
wiley   +1 more source

Intelligent system in the cost control of commercial complex projects: Data-driven optimization method. [PDF]

PLoS One
Wang S, Li Y, Yao D, Li X.
europepmc   +1 more source

Spray‐Dried Engineered Escherichia coli Whole‐Cell Biocatalysts Enable Cell Recycling and In Vivo α‐Ketoglutarate and NADPH Regeneration for Efficient Codeine Manufacture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A recombinant whole‐cell biocatalytic approach provides several advantages over isolated enzymes, such as improved enzyme stability, in vivo cofactor regeneration, and the facilitation of cascade reactions within a single cell. Whole‐cell biocatalysis has gained attention in medicinal opioid production, due to its technical and economic ...
Ali Jahanian, Carlos Horacio Luna‐Flores, Xu Li, Sally L. Gras, Robert E. Speight   +4 more
wiley   +1 more source

A smart compliance validation framework for steel reinforcement in concrete structures using web extension technology. [PDF]

Sci Rep
Nirmalambal U, Natarajan S, Rajprasad J, Arunvivek GK, Gogineni A, Sembeta RY.   +5 more
europepmc   +1 more source