IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong +5 more
wiley +1 more source
Defending non Patrimonial Rights in the Draft of the European Civil Code
The draft of the new Civil Code includes Book I, "About Persons", a special title on protection of non-patrimonial rights by specific legal means (Title V - "Defending non patrimonial rights"), made as a result of development and modernization of ...
Mihaela Aghenitei
doaj
Civilization code and mentality
The essence and content of the concepts «code», «cultural code» and «civilization code» are analyzed in prposed article and it forms the aim of the study. All cultural phenomena, including ethnic, national and religious cultures, civilizations, any pictures of the world created by the mental activity of culture have their own code. Any code is a system
openaire +1 more source
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang +3 more
wiley +1 more source
Les évolutions écologiques des codes civils étrangers : L’exemple du Code civil belge
Présentation des réformes récentes du Code civil belge en lien avec l'Anthropocène : ordre public environnemental, troubles de voisinage mais absence de préjudice ...
de Clippele, Marie-Sophie +1 more
core
What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady +6 more
wiley +1 more source
Quality criteria of involuntary psychiatric admissions - before and after the revision of the civil code in Switzerland. [PDF]
Kieber-Ospelt I +5 more
europepmc +1 more source
Ethical Aspects in the Activity of Civil Servants. Case Study Romania
Preventing corruption and improving the public service management are the main goals of the promotion of ethical standards for the civil servants.
Popa, Florin, Matei, Ani
core
Model‐Enabled Knowledge Transfer Across Cell Lines, Culture Scales and Conditions
ABSTRACT Mechanistic models are central to quantitative understanding and optimization of Chinese hamster ovary (CHO) cell culture processes, but their utility is often restricted by parameter sets calibrated for specific cell lines, scales, or operating conditions.
Luxi Yu +2 more
wiley +1 more source
What are the boundaries of legal guardianship in Alzheimer's disease? An evidence-based update in the context of the Brazilian Civil Code. [PDF]
Sudo FK, Salles AC, de Santiago CR.
europepmc +1 more source

