Results 301 to 310 of about 1,673,815 (341)

Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm

Biotechnology and Bioengineering, EarlyView.
Dash and coworkers have fabricated a small intestine on a chip, which encapsulates the physiology and biofilm of the small intestinal epithelium. The dynamic system showed villus differentiation within 5 days of flow, and the mucus layer and biofilm showed small intestinal characteristics.
Sanat Kumar Dash, Cláudia N. H. Marques, Gretchen J. Mahler   +2 more
wiley   +1 more source

Electrochemical Catheter Hub Operated by a Wearable Micropotentiostat Prevents Acinetobacter baumannii Infection In Vitro

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Intraluminal infection of central venous catheters, used for long‐term treatment, can result in central line‐associated bloodstream infection (CLABSI). These infections can be challenging to prevent and treat due to formation of biofilms within catheter lumens, which shield bacteria from the human immune response and conventional antimicrobial
Majid Al‐Qurahi, Derek Fleming, Won‐Jun Kim, Ibrahim Bozyel, Robin Patel, Haluk Beyenal   +5 more
wiley   +1 more source

A New Aerosol‐Based Photobioreactor for the Cultivation of Cyanobacteria on Luffa

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cyanobacteria are promising organisms for sustainable biotechnology due to their ability to grow photoautotrophically and their wide range of products. Many cyanobacteria grow in the form of biofilms, which is why the development of photobioreactors (PBR) for the cultivation of cyanobacteria in the form of biofilms is of great interest ...
Jonas Kollmen, Andreas Stiefelmaier, Ramtin Mofrad, Dorina Strieth   +3 more
wiley   +1 more source

Altering 15‐Lipoxygenases to 18‐Lipoxygenases and Their Application to the Production of 5,18‐Dihydroxyeicosapentaenoic Acids

Biotechnology and Bioengineering, EarlyView.
15R‐ and 15S‐lipoxygenases (15R‐ and 15S‐LOXs) were engineered into 18R‐ and 18S‐LOXs, respectively. Escherichia coli expressing these enzymes converted eicosapentaenoic acid into 18R‐ and 18S‐hydroxyeicosapentaenoic acids (18R‐ and 18S‐HEPEs), respectively.
Jin Lee, Su‐Hwan Kang, Tae‐Eui Lee, Deok‐Kun Oh   +3 more
wiley   +1 more source

Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus

Biotechnology and Bioengineering, EarlyView.
A cell‐sheet prepared from NIH 3T3 cells was sustained by a pair of glass microneedles above a glass‐bottom dish under the spinning disc confocal microscope equipped with a dual‐view system (left). The cell‐sheet was mechanically stretched by moving one of the microneedles away from the other and the locations of proteins related to focal adhesion were
Madoka Suzuki, Keiko Kawauchi, Hiroaki Machiyama, Hiroaki Hirata, Shin'ichi Ishiwata, Hideaki Fujita   +5 more
wiley   +1 more source

Efficient One‐Step Production of 7S,17S‐ and 10S,17S‐Dihydroxydocosahexaenoic Acids by a Double‐Oxygenating 15S‐Lipoxygenase From Chlamydomonas incerta

Biotechnology and Bioengineering, EarlyView.
Biotransformation pathway from polyunsaturated fatty acids into dihydroxy fatty acids as lipid mediators by Escherichia coli expressing Chlamydomonas incerta 15S‐lipoxygenase with double oxygenating activity. The 15S‐lipoxygenase was the most efficient for the simultaneous production of resolvin D5 (7S,17S‐dihydroxydocosahexaenoic acid, 7S,17S‐DiHDHA ...
Hyun‐Ah Park, Jin Lee, Deok‐Kun Oh
wiley   +1 more source

Temporal Galactose‐Manganese Feeding in Fed‐Batch and Perfusion Bioreactors Modulates UDP‐Galactose Pools for Enhanced mAb Glycosylation Homogeneity

Biotechnology and Bioengineering, EarlyView.
This study investigates the temporal effects of galactose and manganese on the N‐glycosylation of Trastuzumab (mAb) antibody during fed‐batch and perfusion bioprocessing modes to reveal the influence of feeding schedules on mAb N‐glycosylation patterns. These findings will aid in building process control schemes for mAb bioprocessing.
Aron Gyorgypal, Erica Fratz‐Berilla, Casey Kohnhorst, David N. Powers, Shishir P. S. Chundawat   +4 more
wiley   +1 more source

What is Civil Engineering?

Doboku Gakkai Ronbunshu, 1986
openaire   +3 more sources

A Mathematical Model for Determining Probabilistic Design Space in Mesenchymal Stem Cell Passage Culture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT With their many therapeutic functions, mesenchymal stem cells (MSCs) are promising sources for regenerative medicine. However, in the manufacture of MSCs, without a method for exploring the effects of long‐term passage on cell proliferation potentials, the design of passage culture processes is challenging.
Keita Hirono, Yusuke Hayashi, Yuuki Ogawa, Masahiro Kino‐oka, Hirokazu Sugiyama   +4 more
wiley   +1 more source

Microtiter Plate Cultivation Systems Enable Chemically Diverse Metabolic Footprints During Bacterial Natural Product Discovery

Biotechnology and Bioengineering, EarlyView.
The utilization of microtiter plate cultivation systems in the discovery of natural products expands the secondary metabolic footprint. Microtiter plate cultivation systems, in particular the 48 well flower plate, facilitate the parallelization of bacterial cultivation and offer a greater degree of comparability to stirred tank bioreactors in ...
Anton Lindig, Georg Hubmann, Stephan Lütz   +2 more
wiley   +1 more source