Results 241 to 250 of about 1,610,210 (341)

Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been addressed. In this contribution, growth, green fluorescence protein (GFP) expression, and oxygen consumption
Laura Pohlen   +6 more
wiley   +1 more source

Use of Whole Cells and Cell‐Free Extracts of Catalase‐Deficient E. coli for Peroxygenase‐Catalyzed Reactions

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Unspecific peroxygenases (UPOs) and cytochrome P450 monooxygenases (CYPs) with peroxygenase activity are becoming the preferred biocatalysts for oxyfunctionalization reactions. While whole cells (WCs) or cell‐free extracts (CFEs) of Escherichia coli are often preferred for cofactor‐dependent monooxygenase reactions, hydrogen peroxide (H2O2 ...
Ana C. Ebrecht   +5 more
wiley   +1 more source

Bayesian Optimization in Bioprocess Engineering—Where Do We Stand Today?

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Bayesian optimization is a stochastic, global black‐box optimization algorithm. By combining Machine Learning with decision‐making, the algorithm can optimally utilize information gained during experimentation to plan further experiments—while balancing exploration and exploitation.
Florian Gisperg   +5 more
wiley   +1 more source

Biaxial seismic response of base-column connections in sub-standard steel buildings: dataset. [PDF]

open access: yesOpen Res Eur
Stathas N   +8 more
europepmc   +1 more source

High‐Efficiency PET Degradation With a Duo‐Enzyme System Immobilized on Magnetic Nanoparticles

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The widespread consumption of PET worldwide has necessitated the search for environment‐friendly methods for PET degradation and recycling. Among these methods, biodegradation stands out as a promising approach for recycling PET. The discovery of duo enzyme system PETase and MHETase in 2016, along with their engineered variants, has ...
Siddhi Kotnis, Siddhant Gulati, Qing Sun
wiley   +1 more source

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