Results 171 to 180 of about 79,350 (309)

Children's food activism: Reflections on knowledge and responsibility

open access: yesBritish Educational Research Journal, EarlyView.
Abstract Children as activists have become more prominent in recent years. Their activism is manifest in relation to conspicuously political topics such as Black Lives Matter, voting rights, being able to air their views or protesting against injustices related to race, culture and other identities. Less often, if at all, do children engage politically
Sharon Hunter   +7 more
wiley   +1 more source

Organizing Civil Resistance: Understanding the Effects and Dynamics of Organizational Structures on the Outcome of Civil Resistance Campaigns [PDF]

open access: yes
The use of nonviolent tactics has become the most common way to achieve change. The field of civil resistance has been predominantly focused on the dynamics related to the outcome, however, surprisingly little or no attention has been given to ...
Heuver, Lars
core  

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

open access: yesBiotechnology and Bioengineering, EarlyView.
One‐pot liquid‐phase synthesis of the synthetic ligand B1Al2A2 enables controllable ligand density and efficient capture of IgG and IgY with high recovery and purity, streamlining small‐ligand affinity purification workflows. ABSTRACT The development of synthetic affinity ligands offers a cost‐effective alternative to traditional biological ligands ...
Carolina Mota Natal   +8 more
wiley   +1 more source

Numerical and Experimental Assessment of Oxygen Distribution in a Perfusion Bioreactor to Mimic the Osteochondral Niche

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Oxygen supply is a critical parameter in 3D cell cultivation using bioreactors. Since bioreactor designs often prioritise practical constraints, understanding the oxygen supply dynamics of the media is crucial for achieving either uniform or spatially controlled oxygen delivery.
Franziska Alt   +4 more
wiley   +1 more source

A Practical Gut‐On‐Chip Workflow for Imaging‐Based Assessment of Epithelial Barrier Dynamics During E. coli Co‐Culture

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The intestinal epithelium and its resident microbiota form a dynamic interface that is central to gut homeostasis, but this interface is difficult to model with conventional tools. Static Transwell cultures lack physiological fluid shear, whereas animal models are costly and poorly suited to quantitative, high‐content imaging.
Shih‐Wei Chiang   +2 more
wiley   +1 more source

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong   +5 more
wiley   +1 more source

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