Results 221 to 230 of about 1,834,764 (288)

Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy

Biotechnology and Bioengineering, EarlyView.
Schematic depicting the workflow for model building and validation using Raman spectroscopy for real‐time monitoring of IVT. ABSTRACT The success of COVID‐19 mRNA vaccines showcased the transformative potential of this adaptable, plug‐and‐play, and rapid response platform.
Julia Puppin Chaves Fulber, Xingge Xu, Hadi El Radi, Kevin Grollier, Amine A. Kamen   +4 more
wiley   +1 more source

The Liability for Damage Caused by the Use of Artificial Intelligence (AI) in Medicine. [PDF]

Med Arch
Matesa B, Masic I.
europepmc   +1 more source

Genetically Programmed Control of Overflow Metabolism

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen, Andriy Glushchuk, Thilde P. Hjorth, Alvaro R. Lara   +3 more
wiley   +1 more source

A Bourdieusian Approach to the Demobilisation of Brazil's AIDS Movement. [PDF]

Sociol Health Illn
Achcar HM.
europepmc   +1 more source

Advancing Malic Acid Production of Aspergillus oryzae: Combined Process Optimization and Application of Various Substrates

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In light of climate change and growing resource scarcity, microbial production of organic acids offers a sustainable alternative to fossil‐based chemical synthesis. In this study, malic acid production by Aspergillus oryzae was optimized through cultivation temperature adjustment and biotin supplementation, while organic acid formation from ...
Lukas Hartmann, Anke Neumann, Dirk Holtmann, Björn Vater, Thomas Hahn, Susanne Zibek, Katrin Ochsenreither   +6 more
wiley   +1 more source

Social movements are transformative agents for biodiversity conservation. [PDF]

Proc Natl Acad Sci U S A
Walter M, Scheidel A, Vadez C, Bene DD, Temper L, Fanari E, Reyes-García V.   +6 more
europepmc   +1 more source

Establishment of a Single‐Cell Minimal Medium: A Case Study for Microfluidic Cultivation of Corynebacterium glutamicum

Biotechnology and Bioengineering, EarlyView.
Conventional bulk media are often unsuitable for single‐cell cultivation and analysis because they provide excess nutrients or lack essential components. Redesigning the medium allows for controlled, defined growth, enabling the development of a robust minimal medium that supports quantitative and reproducible single‐cell analysis across different ...
Judith Wegmann, Matthias Pesch, Juliane Zwoch, Andreas Schmid, Dietrich Kohlheyer, Alexander Grünberger   +5 more
wiley   +1 more source

Protein Diffusion in Electroporation‐Based Biopsy: Quantifying Protein Transport From Human Skin for Skin Liquid Sampling With Electroporation

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Electroporation‐based biopsy (e‐biopsy) enables minimally invasive extraction of tissue proteins without excision; however, the effective diffusion from tissue into the sampling medium, a key parameter required for this procedure, has not been quantified in human tissues. We coupled time‐resolved aqueous sampling with a one‐dimensional Fickian
Amir Duenyas, Batel Gabay, Ariel Berl, Ofir Shir‐Az, Avshalom Shalom, Martin L. Yarmush, Alexander Golberg   +6 more
wiley   +1 more source

Dignity over DOT: Call to transition from directly observed therapy to rights-based, person-centered care and treatment support. [PDF]

PLOS Glob Public Health
Vo LNQ, Ashesh A, Tcholakov Y, Enkh-Amgalan H, van der Westhuizen HM, members of the WHO Civil Society Task Force on Tuberculosis.   +5 more
europepmc   +1 more source

Raman Spectroscopy–Based Glucose Feed Control Automates Malic Acid Fermentation

Biotechnology and Bioengineering, EarlyView.
Raman spectroscopy enables automated, closed‐loop glucose feeding for malic acid production with Ustilago trichophora. Online PLS regression of glucose and malic acid provides input for PID control to maintain 20 g/L glucose. The feed is dynamically adjusted and stopped in response to metabolic demand and product inhibition, demonstrating practical ...
Luca Antonia Grebe, Katharina Hürter, Dominik Engel, Alexander Walter Wilhelm Echtermeyer, Mafalda S. Santos, Laurin Röder, Valentin Steier, Jörn Viell, Jørgen Barsett Magnus   +8 more
wiley   +1 more source