Results 31 to 40 of about 377,718 (265)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Surface deformation characteristics of shallow buried and rapidly advancing longwall face in Jinjie coal mine

Geomechanics and Geophysics for Geo-Energy and Geo-Resources
In high-intensity mining operations in western China, shallow burial depths and rapid advancement rates induce rapid surface responses, significant deformation, and pronounced crack development.
Xinjie Liu, Anmin He, Xiaobin Li, Xiaolong Wang, Yongqiang Zhao   +4 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Stage-Wise Simulation for Operational Stability Evaluation of Seasonal Heat Storage in Abandoned Coal Mines

Energies
The development of coal resources has created a large number of underground mined-out spaces, which can be utilized for cross-seasonal thermal storage through underground reservoirs to achieve seasonal heat storage.
Wenying Tang, Jiawei Tang, Qiang Guo, Haiqin Zhang, Changhao Feng, Xiaolin He, Zixu Hu, Xi Wu   +7 more
doaj   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Performance Evaluation of Heat Storage in a Full Operation Cycle of Cross-Seasonal Thermal Energy Storage Systems in Coal Mine Underground Reservoirs

Applied Sciences
Coal mining has generated a large amount of underground space, which has traditionally been reused mostly as mine wastewater storage. Given the excellent thermal insulation properties of these mine reservoirs, their potential for seasonal energy storage ...
Wenying Tang, Jiawei Tang, Qiang Guo, Haiqin Zhang, Changhao Feng, Yilong Yuan, Xiaolin He, Zixu Hu   +7 more
doaj   +1 more source

Peningkatan Kualitas Tahu Mbak Trisni Di Kecamatan Percut Sei Tuan

Dinamisia, 2020
The business of Tofu Mbak TRISNI located in Percut Sei Tuan has produced about 2 years. The Problems in the partner's efforts are: 1) The partner has not yet had the knowledge and understanding of the quality of clean water and is worthy tobe used for ...
Herbert Sipahutar, Makmur Sirait, Mukti Hamjah Harahap, Irfandi, Deo Demonta Panggabean, Anggriyani, Dedy Husrizal Syah   +6 more
doaj   +1 more source

Reservoir Perencanaan Struktur Reservoir Air Bersih Desa Pecangaan Kulon, Kecamatan Pecangaan, Kabupaten Jepara

Jurnal Civil Engineering Study, 2022
Keberadaan air bersih di sebuah desa sangat penting, melihat aktivitas kehidupan pedesaan yang sekarang mulai dinamis. Perencanaan ini berdasar pada SNI 7509:2011 tentang Tata Cara Perencanaan Teknik Jaringan Distribusi dan Unit Pelayanan Sistem ...
Herry Puguh Prasetya, Khotibul Umam, Decky Rochmanto   +2 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source