The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. [PDF]
Genes & Development, 1997 Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists of four subunits of 160, 100, 73, and 30 kD. Here we report the isolation and characterization of a cDNA encoding the 30-kD
Silvia M.L. Barabino +4 more
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Cleavage Factor II of Saccharomyces cerevisiaeContains Homologues to Subunits of the Mammalian Cleavage/ Polyadenylation Specificity Factor and Exhibits Sequence-specific, ATP-dependent Interaction with Precursor RNA [PDF]
Journal of Biological Chemistry, 1997 Cleavage of pre-mRNA during 3′-end formation in yeast requires two protein factors, cleavage factor I (CF I) and cleavage factor (CF II). A 5300-fold purification of CF II indicates that four polypeptides of 150, 105, 100, and 90 kDa copurify with CF II ...
Jing Zhao +2 more
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The Cleavage and Polyadenylation Specificity Factor in Xenopus laevis Oocytes Is a Cytoplasmic Factor Involved in Regulated Polyadenylation [PDF]
Molecular and Cellular Biology, 1999 During early development, specific mRNAs receive poly(A) in the cytoplasm. This cytoplasmic polyadenylation reaction correlates with, and in some cases causes, translational stimulation. Previously, it was suggested that a factor similar to the multisubunit nuclear cleavage and polyadenylation specificity factor (CPSF) played a role in cytoplasmic ...
Kirsten S. Dickson +3 more
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Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro. [PDF]
Genes & Development, 1996 We have previously shown that the U1 snRNP-A protein (U1A) interacts with elements in SV40 late polyadenylation signal and that this association increases polyadenylation efficiency.
Carol S. Lutz +5 more
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Poly(A) Tail Length Is Controlled by the Nuclear Poly(A)-binding Protein Regulating the Interaction between Poly(A) Polymerase and the Cleavage and Polyadenylation Specificity Factor [PDF]
Journal of Biological Chemistry, 2009 Poly(A) tails of mRNAs are synthesized in the cell nucleus with a defined length, ∼250 nucleotides in mammalian cells. The same type of length control is seen in an in vitro polyadenylation system reconstituted from three proteins: poly(A) polymerase ...
Uwe Kühn +5 more
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Role of cleavage and polyadenylation specificity factor 100: anchoring poly(A) sites and modulating transcription termination [PDF]
The Plant Journal, 2017 SummaryCPSF100 is a core component of the cleavage and polyadenylation specificity factor (CPSF) complex for 3′‐end formation of mRNA, but it still has no clear functional assignment. CPSF100 was reported to play a role in RNA silencing and promote flowering in Arabidopsis.
Juncheng Lin +4 more
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Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit. [PDF]
Molecular and Cellular Biology, 1994 During the formation of the 3' ends of mRNA, the cleavage and polyadenylation specificity factor (CPSF) is required for 3' cleavage of the transcript as well as for subsequent polyadenylation. Using peptide sequences from a tryptic digest, we have cloned the 100-kDa subunit of CPSF.
Andreas Jenny, H P Hauri, Walter Keller
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Unique Features of Plant Cleavage and Polyadenylation Specificity Factor Revealed by Proteomic Studies [PDF]
Plant Physiology, 2009 AbstractCleavage and polyadenylation of precursor mRNA is an essential process for mRNA maturation. Among the 15 to 20 protein factors required for this process, a subgroup of proteins is needed for both cleavage and polyadenylation in plants and animals.
Hongwei Zhao +2 more
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Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit fromPyrococcus horikoshii [PDF]
Proteins: Structure, Function, and Bioinformatics, 2010 Yuya Nishida +5 more
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The Polyadenylation Factor Subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A Key Factor of Programmed Cell Death and a Regulator of Immunity in Arabidopsis [PDF]
Plant Physiology, 2014 Abstract Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized cell death.
Quentin Bruggeman +8 more
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