Results 121 to 130 of about 7,984 (276)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Interacción clon-espaciamiento en Populus deltoides

, 1998
The objective of this work was to evaluate in Populus deltoides Marshall cultivars USA-Catfish 2 and USA-Stoneville 72 the clon-spacing interaction at different ages. The study was carried out in a systematic spacing experiment, which was located in Alberti, Buenos Aires, Argentina (35º 40' S; 60º 15' W; 52 masl).
Marlats, Raúl M., Bratovich, R., Marquina, Jorge Luis, Arce, J.   +3 more
openaire   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Evaluación de la reacción de nueve genotipos de papa (<em>Solanum tuberosum</em>subsp. <em>andigena</em>) al ataque de <em>Phytophthora infestans</em> (Mont) de Bary

Agronomía Colombiana, 2008
<p align="justify"> El presente trabajo se realizó en el municipio de Pasto, departamento de Nariño, Colombia, a 2.710 msnm. El objetivo fue evaluar la reacción de los clones de papa Blanca, Pintada, Rosada tipo Capiro, Roja Guata, Roja ...
Betancourth G. Carlos, Portilla B. Elizabeth, Salas P. Hugo   +2 more
doaj  

Aristotelia chilensis (mol.) Stuntz. Maqui, maquei, clon, queldron

, 2003
1
Campos-Roasio, Jorge, Sotomayor-Garretón, Álvaro, García-Rivas, Edison, Larrain-Larrain, Oscar, Aguilera-Fernández, Mauricio, Valdebenito-Rebolledo, Gerardo, Ferrando-Toso, Mauro, Kahler-González , Carlos   +7 more
openaire   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Primer reporte de susceptibilidad del clon de caucho natural FX-3864 a Microcyclus ulei en la altillanura colombiana

Revista Colombiana de Biotecnología, 2011
First report of susceptibility of natural rubber clone FX-3864 a Microcyclus ulei en la altillanuera colombiana   Resumen El mal suramericano de las hojas (SALB), enfermedad endémica del caucho (Hevea brasiliensis), es causado por Microcyclus ulei (forma
Ibonne Aydee García-Romero, Olga M. Castro, Fabio Aristizábal, Aníbal L. Tapiero   +3 more
doaj  

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source