Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
Advanced Science, EarlyView.This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Fatal reactions to alpha-gal might be due to comorbid mast cell diseases. [PDF]
J Allergy Clin Immunol PractMoraczewski J, Stone CA.
europepmc +1 more source
Inheritable Epigenetic Memory Induced by Parental Salt Stress Influences Transgenerational Plasticity of <i>Phragmites australis</i>. [PDF]
Ecol EvolChen YH +6 more
europepmc +1 more source
Isolation leads to greater clonality and reduced seed production in a temperate seagrass. [PDF]
Ann BotSmith TM +7 more
europepmc +1 more source
Genomic comparison of clinical strains of <i>Mycobacterium shinjukuense</i> in Japan reveals low diversity and stable genome structures. [PDF]
Microb GenomWada T +8 more
europepmc +1 more source
Modeling the evolutionary dynamics of clonal hematopoiesis. [PDF]
Nat GenetMarzban S +15 more
europepmc +1 more source
Clonal Cocoa Varieties Growth and Leaf Non-Structural Carbohydrate Response to Field Stress Conditions. [PDF]
Plant Environ InteractAidoo MK +4 more
europepmc +1 more source
Mitigating Clonal Variation in Recombinant Mammalian Cell Lines
Trends in Biotechnology, 2019 Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a ...
Jae Seong Lee +2 more
exaly +4 more sources