Results 301 to 310 of about 790,447 (335)
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Diabetes, 1996
The role of T-cells in the pathogenesis of IDDM has been an area of much interest, and investigators have recently acquired new tools for studies on T-cells with the advent of T-cell clones that are reactive with islet antigens. Derived from NOD mice, diabetogenic T-cell lines and clones have for the most part been CD4+ and T-helper 1 (Th1)-like in ...
K, Haskins, D, Wegmann
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The role of T-cells in the pathogenesis of IDDM has been an area of much interest, and investigators have recently acquired new tools for studies on T-cells with the advent of T-cell clones that are reactive with islet antigens. Derived from NOD mice, diabetogenic T-cell lines and clones have for the most part been CD4+ and T-helper 1 (Th1)-like in ...
K, Haskins, D, Wegmann
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Anticytomegalovirus T-Cell Clones
New England Journal of Medicine, 1996To the Editor: The article by Walter et al. (Oct. 19 issue)1 provides important evidence that the infusion of donor–derived CD8+ cytotoxic T-cell clones specific for human cytomegalovirus proteins can promptly reconstitute cellular immunity against human cytomegalovirus in recipients of allogeneic bone marrow, thus reducing the risk of morbidity and ...
Locatelli, F, Maccario, R, Gerna, G
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Clones of alloreactive T cells
Nature, 1978Abstract Previous observations from our lab and many others have suggested that MLR determinants are composed of I region gene products. We extended our original observations by studying the reactivities of responder cells obtained after in vitro primary stimulation (primed responder cells = PRC) with selected stimulator cells.
Fathman, C G, Hengartner, H
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Somatic Cell Cloning without Micromanipulators
Cloning, 2001Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst
Vajta, G. +4 more
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Cloning by function: expression cloning in mammalian cells
Trends in Pharmacological Sciences, 1994malian protein, the most important decision is the selection of a suitable strategy. Until recently, cloning re- quired prior purification of the pro- tein, and the corresponding cDNA was isolated by one of two approaches: (1) Micro-sequencing of all or parts of the purified protein was used to generate nucleic acid probes for ...
H, Simonsen, H F, Lodish
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1987
Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Embryonic and somatic cell cloning
Reproduction, Fertility and Development, 1999Revolutionary opportunities in biology, medicine and agriculture arise from the observation that offspring are obtained after nuclear transfer if somatic donor cells are induced to become quiescent. Exploitation of many of these opportunities will depend upon optimizing procedures for nuclear transfer.
I, Wilmut, L, Young, K H, Campbell
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