Results 71 to 80 of about 4,973,938 (295)

New platform for simple and rapid protein-based affinity reactions

Scientific Reports, 2017
We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest.
Kei Kubota, Takuya Kubo, Tetsuya Tanigawa, Toyohiro Naito, Koji Otsuka   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Waste Algae-Derived Biochar Composites for Synergistic Soil Cd–As Immobilization: Feasibility, Dose–Response Thresholds, and Mechanism

Agronomy
The antagonistic geochemical behaviors of cadmium (Cd) and arsenic (As) in co-contaminated soils complicate their simultaneous remediation. This study aimed to develop a synergistic immobilization strategy by converting Spirulina residue into a magnetic ...
Xue Zhou, Lianfang Li, Mengqi Kang
doaj   +1 more source

Novel Formulations of C-Peptide with Long-Acting Therapeutic Potential for Treatment of Diabetic Complications

Pharmaceutics, 2019
The development and application of novel nanospheres based on cationic and anionic random amphiphilic polypeptides with prolonged stability were proposed. The random copolymers, e.g., poly(l-lysine-co-d-phenylalanine) (P(Lys-co-dPhe)) and poly(l-glutamic
Natalia Zashikhina, Vladimir Sharoyko, Mariia Antipchik, Irina Tarasenko, Yurii Anufrikov, Antonina Lavrentieva, Tatiana Tennikova, Evgenia Korzhikova-Vlakh   +7 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Synthesis and analysis of silica nanocarriers for pectinase immobilization: Enhancing enzymatic stability for continuous industrial applications

Heliyon
Pectinolytic enzymes are among the important group of industrial enzymes that have wide applications in different food industries. In this study, pectinase-based silica nanocarriers were synthesized using co-precipitation and cross-linking techniques ...
Tayyaba Behram, Sidra Pervez, Muhammad Asif Nawaz, Rahim Ullah, Azmat Ali Khan, Bushra Ahmad, Amer M. Alanzai, Asrar Ahmad, Abdul Khaliq Jan, Haneef Ur Rahman, Muhsin Jamal, Tour jan, Abrar Mohyuddin, Nasir Mehmood Khan, Shujaat Ahmad   +14 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Influence of the Immobilization Technique on the Productivity of Enzymes in the Cascade Reduction of CO2 to CH3OH

Advanced Energy & Sustainability Research
The enzymatic effectiveness in the reaction cascade that reduces CO2 to methanol in water at room‐temperature faces various constraints. One of the major challenges is the short life of costly enzymes: immobilization is used to make them more stable and ...
Carmela Di Spiridione, Michele Aresta, Angela Dibenedetto   +2 more
doaj   +1 more source

Immobilization of high-concentration antimony by schwertmannite: Structural incorporation and mineral stability

Chemical Engineering Journal Advances
Antimony (Sb) is a priority pollutant frequently co-occurring with arsenic (As) in mining-impacted environments. The speciation, mobility, and fate of Sb in the environment are intricately linked to its interactions with iron minerals at micro-interfaces.
Yiming Zhang, Zhiwei Sun, Ru Wang, Hua Li, Xiaomeng Wang, Guanyu Zheng   +5 more
doaj   +1 more source