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The enzyme stability of dehydro-enkephalins
Peptides, 1982Dehydro-enkephalins [delta Ala2]-, [delta Ala3]-, [delta Phe4]-, and [delta Leu5]enkephalins, were examined for their stability to enzymatic hydrolysis by carboxypeptidase Y [EC 3.4.16.1]. The successively liberated amino acids were determined quantitatively by amino acid analyses.
Y, Shimohigashi, H C, Chen, C H, Stammer
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Effect of Salts on Enzyme Stability
Annals of the New York Academy of Sciences, 1988Pour tester la stabilite de trois enzymes en presence de solutions salines, les concentrations croissantes de chlorure de sodium, potassium ou lithium sont ajoutees a la preparation enzymatique. La demi-vie de l'enzyme est determinee. La sepctroscopie Raman permet d'etudier le degre d'organisation de l'environnement aqueux et d'expliquer en partie l ...
D, Combes, W N, Ye, A, Zwick, P, Monsan
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Stabilization of food dispersions by enzymes
Food & Function, 2014Food dispersions have become essential vehicles to carry and deliver functional ingredients such as bioactive compounds, flavors, antimicrobials, antioxidants, colors and vitamins. Most of these systems are thermodynamically unstable tending to break down over time.
Benjamin, Zeeb +2 more
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Enzyme stabilization: state of the art
Molecular and Cellular Biochemistry, 1991'Enzyme stabilization' is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships existing in enzymatic molecules.
Gianfreda L, Scarfi' MR
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Stabilization of Enzymes by Using Thermophiles
2017Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana Luisa, Ribeiro +3 more
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Glucosylglycerol and glucosylglycerate as enzyme stabilizers
Biotechnology Journal, 2010AbstractCompatible solutes constitute a diverse class of low‐molecular‐mass organic molecules that are accumulated in high intracellular concentrations in response to the external stress of hyperosmolality or high temperature. Many of these compounds like α, α‐trehalose are well known for their stabilizing effect on protein structure and could lead to ...
Thornthan, Sawangwan +2 more
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Directed evolution of enzyme stability
Biomolecular Engineering, 2005Modern enzyme development relies to an increasing extent on strategies based on diversity generation followed by screening for variants with optimised properties. In principle, these directed evolution strategies might be used for optimising any enzyme property, which can be screened for in an economically feasible way, even if the molecular basis of ...
Vincent G H, Eijsink +3 more
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Enzyme Stabilization by Directed Evolution
2000Engineered enzymes are widely used in industrial processes, where they provide distinct advantages over the naturally-occurring ones (1, 2). Engineering a useful enzyme is particularly difficult when the target functions—for example, the environment in which it must work or the substrates it must accept--differ significantly from the natural ones ...
Gershenson, Anne, Arnold, Frances H.
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Enzyme Stabilization via Cross-Linked Enzyme Aggregates
2010Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in both conventional aqueous media as well as nonaqueous media.
Munishwar N, Gupta, Smita, Raghava
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Introduction to the Field of Enzyme Immobilization and Stabilization
2010Enzyme stabilization is important for many biomedical or industrial application of enzymes (i.e., cell-free biotransformations and biosensors). In many applications, the goal is to provide extended active lifetime at normal environmental conditions with traditional substrates at low concentrations in buffered solutions. However, as enzymes are used for
Michael J, Moehlenbrock +1 more
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