Results 11 to 20 of about 560,659 (266)

A holistic carrier-bound immobilization approach for unspecific peroxygenase

Frontiers in Chemistry, 2022
Unspecific peroxygenases (UPOs) are among the most studied enzymes in the last decade and their well-deserved fame owes to the enzyme’s ability of catalyzing the regio- and stereospecific hydroxylation of non-activated C–H bonds at the only expense of ...
Piera De Santis, Noémi Petrovai, Lars-Erik Meyer, Markus Hobisch, Selin Kara, Selin Kara   +5 more
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Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts

Molecules, 2022
Four commercial immobilized lipases biocatalysts have been submitted to modifications with different metal (zinc, cobalt or copper) phosphates to check the effects of this modification on enzyme features. The lipase preparations were Lipozyme®TL (TLL-IM)
José R. Guimarães, Diego Carballares, Paulo W. Tardioli, Javier Rocha-Martin, Roberto Fernandez-Lafuente   +4 more
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Simple Enzyme Immobilization for Flow Chemistry? An Assessment of Available Strategies for an Acetaldehyde-Dependent Aldolase

Molecules, 2022
Enzyme immobilization is a technology that enables (bio-)catalysts to be applied in continuous-flow systems. However, there is a plethora of immobilization methods available with individual advantages and disadvantages. Here, we assessed the influence of
Martin Wäscher, Thomas Classen, Jörg Pietruszka   +2 more
doaj   +1 more source

Data-driven enzyme immobilisation: a case study using DNA to immobilise galactose oxidase

Engineering Biology, 2020
Biocatalysis has the potential to enable green chemistry. New methods of enzyme immobilisation will be required to improve enzyme stability, product purification, and compatibility of different enzymes in the same reaction conditions.
Wolfgang Ott, Alessandro Ceccarelli, Jack Manning, Nicholas J. Turner, Robert Oppenheimer   +4 more
doaj   +1 more source

The Effect of Calcium Alginate Entrapment on the Stability of Novel Lipases from P. Reinekei and P. brenneri

Trends in Peptide and Protein Sciences, 2019
The high cost of soluble enzymes can limit their use for commercial and industrial purposes. Immobilization can enhance enzyme reusability, thereby reducing product isolation costs and overcoming this economic barrier.
Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan   +3 more
doaj   +1 more source

Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB

Molecules, 2014
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB).
Joana S. Alves, Cristina Garcia-Galan, Mirela F. Schein, Alexandre M. Silva, Oveimar Barbosa, Marco A. Z. Ayub, Roberto Fernandez-Lafuente, Rafael C. Rodrigues   +7 more
doaj   +1 more source

Effect of Concentrated Salts Solutions on the Stability of Immobilized Enzymes: Influence of Inactivation Conditions and Immobilization Protocol

Molecules, 2021
This paper aims to investigate the effects of some salts (NaCl, (NH4)2SO4 and Na2SO4) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three proteases, two glycosidases, and one laccase, penicillin G acylase and ...
Sabrina Ait Braham, El-Hocine Siar, Sara Arana-Peña, Diego Carballares, Roberto Morellon-Sterling, Hossein Bavandi, Diandra de Andrades, Jakub F. Kornecki, Roberto Fernandez-Lafuente   +8 more
doaj   +1 more source

Immobilized Staphylococcus aureus phospholipase C on calcium alginate-chitosan: improved performance and industrial potential in soybean oil refining

Frontiers in Bioengineering and Biotechnology
Phospholipases are versatile biocatalysts with wide applications in the food and oil industries due to their ability to hydrolyze phospholipids and improve product quality. In this study, production of an extracellular phospholipase C from Staphylococcus
Areej Ali Alzahrani, Najeh Krayem, Mona Alonazi, Eman Al-Shehri, Habib Horchani, Abir Ben Bacha   +5 more
doaj   +1 more source

Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation

Molecules
In the glycerolysis process for diacylglycerol (DAG) preparation, free lipases suffer from poor stability and the inability to be reused. To address this, a cost-effective immobilized lipase preparation was developed by cross-linking macroporous resin ...
Rui Xie, Yee-Ying Lee, Pengkai Xie, Chin-Ping Tan, Yong Wang, Zhen Zhang   +5 more
doaj   +1 more source

Enantiomeric Tartaric Acid Production Using cis-Epoxysuccinate Hydrolase: History and Perspectives

Molecules, 2019
Tartaric acid is an important chiral chemical building block with broad industrial and scientific applications. The enantioselective synthesis of l(+)- and d(−)-tartaric acids has been successfully achieved using bacteria presenting cis ...
Jinsong Xuan, Yingang Feng
doaj   +1 more source