Results 11 to 20 of about 989,780 (312)
Investigating on the Stability of Peroxidase Extracted from Soybean (Glycine max var. Williams) and Effects of Na+ and K+ Ions on its Activity [PDF]
Journal of Cell and Molecular Research, 2015 In the present study, some techniques were used for Soybean peroxidase (SBP) purification including: ammonium sulfate fractionation, DEAE Sephadex anion exchange chromatography and Concanavalin A Sepharose 4B affinity chromatography.
Leila Parsiavash +2 more
doaj +1 more source
A holistic carrier-bound immobilization approach for unspecific peroxygenase
Frontiers in Chemistry, 2022 Unspecific peroxygenases (UPOs) are among the most studied enzymes in the last decade and their well-deserved fame owes to the enzyme’s ability of catalyzing the regio- and stereospecific hydroxylation of non-activated C–H bonds at the only expense of ...
Piera De Santis +5 more
doaj +1 more source
Assaying activity and assessing thermostability of hyperthermophilic enzymes [PDF]
, 2001 There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature.
Akanuma +22 more
core +2 more sources
Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide [PDF]
, 2007 Horseradish peroxidase (HRP) is a commonly used enzyme in many biotechnological fields. Improvement of HRP stability would further increase its potential application range.
Arnold +18 more
core +2 more sources
Molecules, 2022 Four commercial immobilized lipases biocatalysts have been submitted to modifications with different metal (zinc, cobalt or copper) phosphates to check the effects of this modification on enzyme features. The lipase preparations were Lipozyme®TL (TLL-IM)
José R. Guimarães +4 more
doaj +1 more source
Astroenzymology – the environmental limits of enzyme activity [PDF]
, 2003 Using organisms from extreme terrestrial environments as models for extraterrestrial life may lead us to underestimate the range of environments that life may inhabit.
Daniel, Roy M.
core +2 more sources
Molecules, 2022 Enzyme immobilization is a technology that enables (bio-)catalysts to be applied in continuous-flow systems. However, there is a plethora of immobilization methods available with individual advantages and disadvantages. Here, we assessed the influence of
Martin Wäscher +2 more
doaj +1 more source
Practical insights on enzyme stabilization [PDF]
Critical Reviews in Biotechnology, 2017 Enzymes are efficient catalysts designed by nature to work in physiological environments of living systems. The best operational conditions to access and convert substrates at the industrial level are different from nature and normally extreme. Strategies to isolate enzymes from extremophiles can redefine new operational conditions, however not always ...
Silva, Carla +4 more
openaire +3 more sources
Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH [PDF]
, 2001 The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C.
Hägerdal B. +6 more
core +2 more sources
Data-driven enzyme immobilisation: a case study using DNA to immobilise galactose oxidase
Engineering Biology, 2020 Biocatalysis has the potential to enable green chemistry. New methods of enzyme immobilisation will be required to improve enzyme stability, product purification, and compatibility of different enzymes in the same reaction conditions.
Wolfgang Ott +4 more
doaj +1 more source