Activity-Stability Relationships in Extremophilic Enzymes [PDF]
Journal of Biological Chemistry, 2003 Psychrophilic, mesophilic, and thermophilic alpha-amylases have been studied as regards their conformational stability, heat inactivation, irreversible unfolding, activation parameters of the reaction, properties of the enzyme in complex with a transition state analog, and structural permeability.
D'Amico, Salvino +3 more
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A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]
, 2004 Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M. +4 more
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Trends in Peptide and Protein Sciences, 2019 The high cost of soluble enzymes can limit their use for commercial and industrial purposes. Immobilization can enhance enzyme reusability, thereby reducing product isolation costs and overcoming this economic barrier.
Priyanka Priyanka +3 more
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Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 [PDF]
, 2005 A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43
A. Rahman, R +7 more
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Characterization of the novel ene reductase Ppo-Er1 from paenibacillus polymyxa [PDF]
, 2020 Ene reductases enable the asymmetric hydrogenation of activated alkenes allowing the manufacture of valuable chiral products. The enzymes complement existing metal- and organocatalytic approaches for the stereoselective reduction of activated C=C double ...
Aregger, David +2 more
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Drug Delivery, 2022 The lipophilicity of a peptide drug can be considerably increased by hydrophobic ion pairing with amphiphilic counterions for successful incorporation into lipid-based formulations.
Yoon Tae Goo +8 more
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Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C [PDF]
, 1996 An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q chromatography) with a yield of 4%.
Daniel, Roy M. +2 more
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Design of surface-active artificial enzyme particles to stabilize Pickering emulsions for high-performance biphasic biocatalysis [PDF]
, 2015 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Surface-active artificial enzymes (SAEs) are designed and constructed by a general and novel strategy.
Baciocchi +39 more
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Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits [PDF]
, 2017 An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon.
Isla, Maria Ines +5 more
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Molecules, 2014 It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB).
Joana S. Alves +7 more
doaj +1 more source