Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid [PDF]
Bacteriophage P22 serves as a model for the assembly and maturation of other icosahedral double-stranded DNA viruses. P22 coat and scaffolding proteins assemble in vitro into an icosahedral procapsid, which then expands during DNA packaging (maturation).
Abendon+53 more
core +1 more source
Distinct stages in the recognition, sorting, and packaging of proTGFα into COPII-coated transport vesicles. [PDF]
In addition to its role in forming vesicles from the endoplasmic reticulum (ER), the coat protein complex II (COPII) is also responsible for selecting specific cargo proteins to be packaged into COPII transport vesicles.
Schekman, Randy, Zhang, Pengcheng
core +2 more sources
Bacteriophages and their structural organisation [PDF]
Viruses are extremely small infectious particles that are not visible in a light microscope, and are able to pass through fine porcelain filters. They exist in a huge variety of forms and infect practically all living systems: animals, plants ...
Orlova, Elena
core +2 more sources
A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence [PDF]
Long RNAs often exist as multiple conformers in equilibrium. For the genomes of single-stranded RNA viruses, one of these conformers must include a compacted state allowing the RNA to be confined within the virion.
Alexander Borodavka+8 more
core +1 more source
Portal protein functions akin to a DNA-sensor that couples genome-packaging to icosahedral capsid maturation. [PDF]
Tailed bacteriophages and herpesviruses assemble infectious particles via an empty precursor capsid (or \u27procapsid\u27) built by multiple copies of coat and scaffolding protein and by one dodecameric portal protein.
Afonine, Pavel V.+7 more
core +2 more sources
The full coat protein gene of Tomato spotted wilt virus (TSWV) was subcloned into a prokaryotic expression vector pET-32a. Results of restriction enzyme digestion and DNA sequencing show that the insertion direction and sequence were correct and no ...
YU Cui+3 more
doaj +1 more source
Designing and evaluation of ELISA kit based on preparing antibodies against Apple stem grooving virus coat protein gene expressed in Escherichia coli [PDF]
Goal: production of antibodies against the coat protein of Apple stem grooving virus and their application in diagnostic assays and biosensor design represents a crucial strategy for effective plant virus detection.
Mahsa Mohammadloo+3 more
doaj +1 more source
Coat proteins isolated from clathrin coated vesicles can assemble into coated pits. [PDF]
Isolated human fibroblast plasma membranes that were attached by their extracellular surface to a solid substratum contained numerous clathrin coated pits that could be removed with a high pH buffer (Moore, M.S., D.T. Mahaffey, F.M. Brodsky, and R.G.W. Anderson. 1987. Science [Wash. DC]. 236:558-563).
Richard G.W. Anderson+3 more
openaire +3 more sources
Restoration of Wild-Type Virus by Double Recombination of Tombusvirus Mutants with a Host Transgene
Nicotiana benthamiana plants transformed with the coat protein gene of tomato bushy stunt virus (TBSV) failed to elicit effective virus resistance when inoculated with wild-type virus.
Marise Borja+3 more
doaj +1 more source
Phylogenetic analysis of Iranian Cucumber mosaic virus isolates from Kerman and Yazd provinces [PDF]
Cucumber mosaic virus (CMV) is one of the most important viruses of cucurbits worldwide. To study the phylogenetic relationships of several CMV isolates, a number of samples were collected from Kerman and Yazd provinces.
Mohammad Maddahian+3 more
doaj +1 more source