Results 81 to 90 of about 405,994 (233)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Near-Infrared-Induced Hydrophobic Characteristic of Black TiO₂ Coatings Showing Antibacterial and Immunomodulatory Properties

Journal of Functional Biomaterials
Surface wettability is a critical factor influencing the biological performance of orthopedic Ti implants. The native TiO2 film on Ti undergoes changes in wettability under ultraviolet (UV) irradiation. However, the limited tissue penetration of UV light
Yulin Gao, Kai Li, Qiang Chen, Pingtuo Wang, Aoshuang Xun, Yi Ding, Heng Ji, Xuebin Zheng   +7 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Simulating the heating procedure of HREE-coated Nd-Fe-B magnets to assess the feasibility realizing GBDP online

Case Studies in Thermal Engineering
To date, the grain boundary diffusion process (GBDP) of heavy rare earth elements (HREEs), such as Dy and Tb, into sintered Nd-Fe-B magnets has been the most efficient approach for improving the coercive force of the magnets.
Xi Zhang, Lei Lu, Zhongdi Lu, Panlong Wang, Guangke Tian   +4 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Diamond-like carbon coating of alternative metal alloys for medical and surgical applications [PDF]

, 2010
The effectiveness of a plasma-deposited, diamond-like carbon (DLC) coating on aluminium alloy based surgical instruments is investigated. Surgical instruments must satisfy a number of important criteria including biocompatibility, functional performance,
Bulpett, R, Reynolds, AJ, Franks, J, Jones, Benjamin, Anson, AW, Jones, B J, Anson, A W, Jones, BJ, Mahendran, A, Reynolds, A J   +9 more
core   +1 more source

Environmentally Friendly R2R Process for Flexible Perovskite Solar Cells via DMF‐Free Cosolvent Ink

EcoMat
Roll‐to‐roll (R2R) coating is an essential manufacturing strategy for the scalable production of perovskite solar cells (PSCs), offering high throughput and compatibility with flexible substrates.
Geon Yeong Park, Hyeji Han, Min‐Jae Kim, Nara Han, Yong Cheon Park, Min Kim, Jongchul Lim, Dong Seok Ham   +7 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source