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Oligodendrocyte–Neuron Myelinating Coculture
2019The preparation of oligodendrocytes and neurons independently in vitro has provided substantial insight into the biology of the process of myelin sheath formation. This chapter describes a myelination system of dorsal root ganglion neurons by independent isolation of oligodendrocyte progenitor cells from either rat or mouse cortex.
Swire, Matthew +1 more
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Coculturing Neurons and Glial Cells
Current Protocols in Toxicology, 2003AbstractThe close relationship between neurons, glia, astroglia, and astrocytes plays a relevant role in modulating a neurotoxic effect and propagating the consequent degenerative event in the brain. After exposure to a neurotoxicant, glial cells are often activated to release substances responsible for the propagation of the damage.
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Development of a Coculture Model of Encapsulated Cells
Annals of the New York Academy of Sciences, 2001Abstract: In the whole animal, metabolic regulations are set by reciprocal interactions between various organs, via the blood circulation. At present, analyses of such interactions require numerous and uneasily controlled in vivo experiments. In a search for an alternative to in vivo experiments, our work aims at developing a coculture system in which
Canaple L +4 more
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Preparation and Coculture of Neurons and Glial Cells
Current Protocols in Cell Biology, 2006AbstractThe close relationship between neurons, glia, astroglia, and astrocytes plays a relevant role in modulating a neurotoxic effect and propagating the consequent degenerative event in the brain. After exposure to a neurotoxicant, glial cells are often activated to release substances responsible for the propagation of the damage.
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Peroxynitrite Exposure of Cells Cocultured with Macrophages
Annals of Biomedical Engineering, 2004Peroxynitrite is a potent oxidant that is formed endogenously from NO and O2-. One approach for studying its toxicity involves the coculture of activated macrophages (producers of NO and O2-) with "target cells" (which make O2- but not NO). Because peroxynitrite concentrations in such experiments are too small and localized to measure, reaction ...
Nitesh, Nalwaya, William M, Deen
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Limitation of syntrophic coculture growth by the acetogen
Biotechnology and Bioengineering, 2015ABSTRACTThe syntrophic cooperation between hydrogen‐producing acetogens and hydrogenotrophic methanogens relies on a critical balance between both partners. A recent study, provided several indications for the dependence of the biomass‐specific growth rate of a methanogenic coculture on the acetogen.
Helena, Junicke +3 more
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The effect of coculture on human zygote development
Current Opinion in Obstetrics and Gynecology, 1993It is well over a decade since the birth of the first test-tube baby and still the in-vitro conditions for early embryonic development remain suboptimal. The ideal culture medium to increase longevity and improve viability of human embryos is not available. Since the metabolic requirements of the human embryo changes from one cleavage stage to another,
A, Bongso, C Y, Fong
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Coculture Methodologies for the Study of Wnt Signals
2008In vivo, responses to extracellular Wnt ligands are context dependent; the temporal characteristics and intensity of the signal are critical in determining the target cell response. In general, Wnt ligand-induced differentiation in mammalian cells requires several days of exposure. In order to better characterize Wnt-induced signaling in vitro, side-by-
Kestutis, Planutis +2 more
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Astrocyte–Schwann-Cell Coculture Systems
2011Schwann cells are one of the cellular candidates used in repair strategies following trauma and demyelination of the spinal cord. One of the major obstacles in the use of Schwann cells is their limited migratory ability within the astrocytic environment of the CNS and boundary formation between the Schwann cells of the graft and the host astrocytes ...
Fardad T, Afshari, James W, Fawcett
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Embryonic chick cocultures of neuronal and muscle cells
Neurological Research, 2008The aim of this report was to set up an effective experimental model of cocultures between cells from spinal cord explants and myotubes from adductor muscle.We obtained neuronal cells from chick spinal cord explants at embryonic day 5 (ED5) by means of an enzymatic digestion.
MARTINELLO, TIZIANA +1 more
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