New developments in coherent anti-Stokes Raman scattering (CARS) microscopy
Frontiers in Optics, 2003Recent advances have improved the capability of CARS microscopy for imaging live cells with high sensitivity. In this contribution an overview of the developments in CARS microscopy is given along with applications to cell biology.
Eric O. Potma +3 more
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A detailed description of a common path interferometer is given for CARS and SRS applications. In this interferometer, both probe and reference arms are separated in time and polarization by a birefringent crystal. In order to generate the pump pulse a Ti: Sa laser centered at 808 nm is used, as for the Stokes pulses a Nd: YVO centered at 1064 nm is ...
Negrete-González, G. +3 more
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Coherent Anti-Stokes Raman Scattering (CARS) And Raman Pumping Lineshapes In High Fields
SPIE Proceedings, 1988We have used an injection-locked Nd:YAG laser/dye laser combination together with a frequency-narrowed argon-fluoride laser to simultaneously observe coherent anti-Stokes Raman scattering (CARS) and stimulated. Raman pumping of oxygen. At high power densities, strong Raman pumping occurs leading to significant distortion of the CARS spectrum and a ...
R B. Miles +3 more
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Experimental study of a CO2 laser plasma by coherent anti-Stokes Raman scattering (CARS)
Spectrochimica Acta Part B: Atomic Spectroscopy, 1992Abstract We have studied the temperature characteristics of the radio frequency excited plasma in a CO 2 waveguide laser by the coherent anti-Stokes Raman scattering (CARS). CARS-spectra of the pumping gas N 2 obtained in (broadband) multiplex technique allowed the spectroscopic determination of the population density in the vibrational states of ...
Kishimoto, T. +4 more
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Coherent Anti-Stokes Raman Scattering (CARS) Microscopy Instrumentation and Applications
1998Abstract Key to the success of optical microscopy in cellular biology is the ability to detect structures with a clear chemical contrast. Identifying the distribution and dynamics of targets in the cell is accomplished only if these compounds can be visualized selectively.
Eric O Potma, X Sunney Xie
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Fiber-Coupled Picosecond Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy
Lasers, Sources and Related Photonic Devices, 2010A proof-of-principle fiber-coupled ps-CARS using multimode step-index fiber is designed and demonstrated for high-temperature gas-phase thermometry in flame. The impact of fiber propagation on the delivered beam and resultant effect on CARS signal is investigated.
Paul S. Hsu +5 more
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Hyperspectral microscopic imaging by multiplex coherent anti-Stokes Raman scattering (CARS)
SPIE Proceedings, 2011Coherent anti-Stokes Raman scattering (CARS) microscopy is a powerful technique to image the chemical composition of complex samples in biophysics, biology and materials science. CARS is a four-wave mixing process. The application of a spectrally narrow pump beam and a spectrally wide Stokes beam excites multiple Raman transitions, which are probed ...
Alexander Khmaladze +8 more
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Towards an integrated coherent anti-Stokes Raman scattering (CARS) microscopy system
SPIE Proceedings, 2005CARS microscopy has emerged as a powerful tool in imaging of biological matter. In addition to a high-3D spatial resolution, the technique delivers an attractive set of properties such as chemical specificity, high sensitivity, and fast data acquisition rates thus making it very suitable for biomedical applications.
Francois Legare +2 more
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Chemical Imaging of Photoresists with Coherent Anti-Stokes Raman Scattering (CARS) Microscopy
The Journal of Physical Chemistry B, 2003Coherent anti-Stokes Raman scattering (CARS) microscopy is demonstrated to be a powerful imaging technique with chemical specificity for studying chemically amplified polymer photoresists. Samples of poly(tert-butyloxycarbonyloxystyrene) (PTBOCST) resist imprinted by interferometric lithography with a pattern of lines/spaces of 400 nm/400 nm and 200 nm/
Eric O. Potma +8 more
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Random illumination coherent anti-Stokes Raman scattering microscopy (RIM-CARS)
Optica Imaging Congress 2024 (3D, AOMS, COSI, ISA, pcAOP)Coherent non-linear wide-field imaging reduces phototoxicity compared to focused approaches and holds the potential for the highest possible image acquisition rates, but misses optical sectioning. Here, we reintroduce optical sectioning using fast changing speckle illuminations.
Eric M. Fantuzzi +6 more
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