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Cold-adapted enzymes: mechanisms, engineering and biotechnological application
Bioprocess and Biosystems Engineering, 2023Most cold-adapted enzymes display high catalytic activity at low temperatures (20-25 °C) and can still maintain more than 40-50% of their maximum activity at lower temperatures (0-10 °C) but are inactivated after a moderate increase in temperature.
Yan, Liu +5 more
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Cold Adaptation of Enzyme Reaction Rates
Biochemistry, 2008A major issue for organisms living at extreme temperatures is to preserve both stability and activity of their enzymes. Cold-adapted enzymes generally have a reduced thermal stability, to counteract freezing, and show a lower enthalpy and a more negative entropy of activation compared to mesophilic and thermophilic homologues.
Sinisa, Bjelic +2 more
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Cold-adapted enzymes: from fundamentals to biotechnology
Trends in Biotechnology, 2000Psychrophilic enzymes produced by cold-adapted microorganisms display a high catalytic efficiency and are most often, if not always, associated with high thermosensitivity. Using X-ray crystallography, these properties are beginning to become understood, and the rules governing their adaptation to cold appear to be relatively diverse.
Gerday, Charles +13 more
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Psychrophilic enzymes: hot topics in cold adaptation
Nature Reviews Microbiology, 2003More than three-quarters of the Earth's surface is occupied by cold ecosystems, including the ocean depths, and polar and alpine regions. These permanently cold environments have been successfully colonized by a class of extremophilic microorganisms that are known as psychrophiles (which literally means cold-loving).
Feller, Georges, Gerday, Charles
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2000
The number of reports on enzymes from cold adapted organisms has increased significantly over the past years, and reveals that adaptive strategies for functioning at low temperature varies among enzymes. However, the high catalytic efficiency at low temperature seems, for the majority of cold active enzymes, to be accompanied by a reduced thermal ...
A O, Smalås +3 more
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The number of reports on enzymes from cold adapted organisms has increased significantly over the past years, and reveals that adaptive strategies for functioning at low temperature varies among enzymes. However, the high catalytic efficiency at low temperature seems, for the majority of cold active enzymes, to be accompanied by a reduced thermal ...
A O, Smalås +3 more
openaire +2 more sources
Computation of enzyme cold adaptation
Nature Reviews Chemistry, 2017Enzymes that are optimized for low temperatures have characteristics that are distinct from those that operate under milder conditions. This Review examines cold-adapted enzymes and describes how computational studies have highlighted structural and energetic consequences…
Johan Åqvist +2 more
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Cold-Adapted Enzymes from Marine Antarctic Microorganisms
Marine Biotechnology, 2006The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the viscosity of the medium, which is also significantly enhanced by low temperatures, contributes to slow down reaction rates. This review analyses the different
Marx, J. C. +4 more
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2001
In the last few years, increased attention has been focused on enzymes produced by cold-adapted micro-organisms. It has emerged that psychrophilic enzymes represent an extremely powerful tool in both protein folding investigations and for biotechnological purposes. Such enzymes are characterised by an increased thermosensitivity and, most of them, by a
D. Georlette +12 more
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In the last few years, increased attention has been focused on enzymes produced by cold-adapted micro-organisms. It has emerged that psychrophilic enzymes represent an extremely powerful tool in both protein folding investigations and for biotechnological purposes. Such enzymes are characterised by an increased thermosensitivity and, most of them, by a
D. Georlette +12 more
openaire +1 more source
Trends in Biotechnology, 1997
It is an article of faith among biochemists and molecular biologists that precious enzymes must be stored on ice. The usual reason given is that, at temperatures around freezing, enzyme activity is minimized and protein stability maximized. There is considerable evidence supporting this, but is it true for all enzymes? What about enzymes from organisms
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It is an article of faith among biochemists and molecular biologists that precious enzymes must be stored on ice. The usual reason given is that, at temperatures around freezing, enzyme activity is minimized and protein stability maximized. There is considerable evidence supporting this, but is it true for all enzymes? What about enzymes from organisms
openaire +2 more sources
Cold Adaptation of the Thermophilic Enzyme 3-Isopropylmalate Dehydrogenase
Journal of Biochemistry, 2001We have performed random mutagenesis coupled with selection to isolate mutant enzymes with high catalytic activities at low temperature from thermophilic 3-isopropylmalate dehydrogenase (IPMDH) originally isolated from Thermus thermophilus. Five cold-adapted mutant IPMDHs with single-amino-acid substitutions were obtained and analyzed. Kinetic analysis
M, Yasugi +4 more
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