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Industrial applications of cold-adapted enzymes: challenges, innovations and future perspective
3 Biotech, 2021Extreme cold environments are potential reservoirs of microorganisms producing unique and novel enzymes in response to environmental stress conditions. Such cold-adapted enzymes prove to be valuable tools in industrial biotechnology to meet the increasing demand for efficient biocatalysts.
Anil Kumar +2 more
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2001
In the last few years, increased attention has been focused on enzymes produced by cold-adapted micro-organisms. It has emerged that psychrophilic enzymes represent an extremely powerful tool in both protein folding investigations and for biotechnological purposes. Such enzymes are characterised by an increased thermosensitivity and, most of them, by a
D. Georlette +12 more
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In the last few years, increased attention has been focused on enzymes produced by cold-adapted micro-organisms. It has emerged that psychrophilic enzymes represent an extremely powerful tool in both protein folding investigations and for biotechnological purposes. Such enzymes are characterised by an increased thermosensitivity and, most of them, by a
D. Georlette +12 more
openaire +1 more source
Cold‐active enzymes from cold‐adapted bacteria
Journal of the American Oil Chemists' Society, 1997AbstractThe properties of amylase, lipase and protease, excreted by newly isolated bacteria from snow‐covered soil, salmon intestine and crab intestine, have been investigated. One amylase, one lipase, and three proteases have been characterized by shifts in their apparent optimal activities toward low temperatures and by reductions in their activation
Yasutaka Morita +5 more
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Cold Adaptation of the Thermophilic Enzyme 3-Isopropylmalate Dehydrogenase
Journal of Biochemistry, 2001We have performed random mutagenesis coupled with selection to isolate mutant enzymes with high catalytic activities at low temperature from thermophilic 3-isopropylmalate dehydrogenase (IPMDH) originally isolated from Thermus thermophilus. Five cold-adapted mutant IPMDHs with single-amino-acid substitutions were obtained and analyzed. Kinetic analysis
M, Yasugi +4 more
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Cold-Active Enzymes from Cold-Adapted Yeasts
2017Cold-adapted yeasts include psychrophiles or psychrotolerant nonconventional species able to survive and grow at low temperatures. They represent an important source of biological diversity that has developed a set of structural and functional adaptation strategies to overcome the adverse effects of cold (sometimes associated with other limiting ...
Marcelo Baeza +4 more
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Cold adaption of enzymes: Structural comparison between salmon and bovine trypsins
Proteins: Structure, Function, and Bioinformatics, 1994AbstractThe crystal structure of an anionic form of salmon trypsin has been determined at 1.82 Å resolution. We report the first structure of a trypsin from a phoikilothermic organism in a detailed comparison to mammalian trypsins in order to look for structural rationalizations for the cold‐adaption features of salmon trypsin.
A O, Smalås +4 more
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Isolation and characterization of psychrophilic yeasts producing cold-adapted pectinolytic enzymes
Letters in Applied Microbiology, 2004The present study was conducted to screen for psychrophilic yeasts that are able to degrade pectin compounds at low temperature, and to examine the cold-active pectinolytic enzymes produced by the isolated psychrophilic yeasts.Psychrophilic yeasts, which grow on pectin as a sole carbon source, pectinolytic-psychrophilic yeast (PPY) strains PPY-3, 4, 5 ...
T, Nakagawa +4 more
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Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts
Extremophiles, 2003The potential of 89 culturable cold-adapted isolates from uncontaminated habitats, including 61 bacterial and 28 yeast strains, to utilize representative fractions of petroleum hydrocarbons (n-alkanes, monoaromatic and polycyclic aromatic hydrocarbons) for growth and to produce various enzymes at 10 degrees C was investigated.
Rosa, Margesin +4 more
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Biotechnology of enzymes from cold-adapted microorganisms
1999One of the main goals in enzyme research is industrial application. Nowadays, we are surrounded by enzymes as well as chemicals produced by enzymes in our daily life. Since papain (EC 3.4.22.2) was used, probably as the first exogenous enzyme, to prevent the formation of chill hazes in beer,1 many enzymes isolated from various species have been ...
S. Ohgiya +4 more
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Complete genome sequence of cold-adapted enzyme producing Microbulbifer thermotolerans DAU221
Journal of Biotechnology, 2016Microbulbifer thermotolerans DAU221 was preliminary isolated from the marine sediment samples in the Republic of Korea. Here, we present the complete genome sequence of M. thermotolerans DAU221, which consisted of 3,938,396 base pairs with a GC content of 56.57%. This genomic information should help us find the industrially useful enzymes.
Yong-Suk, Lee, Yong-Lark, Choi
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