Results 191 to 200 of about 6,280 (209)
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Lethal lysogenization by coliphage lambda
Virology, 1976Abstract Mutants of lambda insensitive to epistatic replication inhibition by repressor ( ri c ) were studied during lysogenization of sensitive host cells. These mutants establish defective complexes which neither lyse nor multiply as lysogens.
Masako Ohashi, William F. Dove
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Nature, 1973
VIRUSES are thought to have either RNA or DNA as their genetic material, but not both1, although oncogenic RNA viruses containing reverse transcriptase have been shown to contain some DNA. I did not expect to find that coliphage T5, a DNA-containing virus, also contained some RNA.
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VIRUSES are thought to have either RNA or DNA as their genetic material, but not both1, although oncogenic RNA viruses containing reverse transcriptase have been shown to contain some DNA. I did not expect to find that coliphage T5, a DNA-containing virus, also contained some RNA.
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Structural proteins of coliphage N4
Virology, 1973Abstract The proteins from bacteriophage N4, dissociated with 1% SDS and 2% 2-mercaptoethanol, have been investigated by SDS polyacrylamide gel electrophoresis. Ten different polypeptide chains were identified in the purified virus. The molecular weights of the protein monomers estimated by coelectrophoresis with marker proteins ranged from 31,000 to
M. Giraldi, Gian Carlo Schito, M. Toni
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Coliphage survival in seawater
Water Research, 1985Abstract The relative survival of coliphages isolated from seawater under controlled conditions in different media, in the laboratory and in the marine environment, was investigated. The results show that the survival under controlled conditions in different media, and also in the sea, is affected by biological and chemical factors.
Juan J. Borrego, Pedro Romero
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The genetics and physiology of coliphage N4
Virology, 1973Amber mutants of bacteriophage N4 were isolated and classified into 27 cistrons by complementation tests. A group of 5 cistrons are essential for the synthesis of phage DNA, whereas 22 other cistrons, when defective, permit varying degrees of N4 DNA replication.
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Molecular weights of coliphages and coliphage DNA
Journal of Molecular Biology, 1970Abstract The range of usefulness of the high-speed equilibrium centrifugation method of Yphantis (1964) has been extended to measure the molecular weight of Escherichia coli phage T7. Values for \ gn of phages T7, T5 and T4 were obtained by pycnometry; the phage concentrations were determined by measuring nitrogen and phosphorus contents, and ...
Frank C. Bancroft, David Freifelder
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In vitro initiation of coliphage T7 mRNA
Archives of Biochemistry and Biophysics, 1972Abstract The synthesis of two classes of late T7 proteins appears to be directed by several polycistronic messenger RNAs. To understand how they are translated in appropriate order during phage infection, we have isolated late mRNA from T7-infected cells and mRNA that was synthesized in vitro with the T7 RNA polymerase and T7 DNA.
Philip Leder, Robert Callahan
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Virology, 1979
Abstract The phage mutant λ nu lt16, which is defective in DNA packaging, defines a new λ gene, nu 1. This has been shown by its ability to complement and be complemented by λ mutants defective in all of the other morphogenetic genes. The nu lt 16 mutation is a 1.28-kb DNA insertion located to the left of gene A , the leftmost known cistron on ...
Robert A. Weisberg+2 more
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Abstract The phage mutant λ nu lt16, which is defective in DNA packaging, defines a new λ gene, nu 1. This has been shown by its ability to complement and be complemented by λ mutants defective in all of the other morphogenetic genes. The nu lt 16 mutation is a 1.28-kb DNA insertion located to the left of gene A , the leftmost known cistron on ...
Robert A. Weisberg+2 more
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Cyclization of coliphage 186 DNA
Journal of Molecular Biology, 1967Equilibrium and kinetic studies have been made on the cyclization of coliphage 186 DNA. In 0·13 m -Na + , the melting temperature of the cohesive ends ( T m ), i.e. the midpoint of the linear monomer to cyclic monomer transition, occurs at 63°C, which is 12°C above that of λb2b5c DNA in the same medium.
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Physical map of coliphage N4 DNA
Virology, 1980Abstract A detailed restriction endonuclease map of Escherichia coli phage N4 DNA is presented. The cleavage sites for restriction endonucleases Hae II (1 cut), Hha I (3 cuts), Xba I (5 cuts), Ava II (15 cuts), and Hpa I (25 cuts) have been determined by (1) digestion with endonucleases after end labeling of the DNA; (2) digestion with two ...
C. Malone+2 more
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