Results 271 to 280 of about 178,057 (307)
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A colorimetric method for the assay of lipoyl dehydrogenase
Mikrochimica Acta, 1975A colorimetric method for the direct assay of lipoyl dehydrogenase is described. Enzyme reaction is stopped by ethanol precipitation. This is followed by the displacement of 1,3-bis(2′-pyridyl)-1,2-diaza-prop-2-ene (PAPHY) from a Pd(II)-PAPHY complex by reduced lipoic acid.
A M, Seet, K T, Lee
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A rapid colorimetric assay for heparinase activity
Analytical Biochemistry, 1991A rapid, sensitive, assay for enzymes that degrade heparin is described. The procedure is based on the interference of heparin with color development during the interaction of protein with the dye Coomassie brilliant blue. The loss of this property when the glycosaminoglycan is degraded by heparinase can be used to quantify activity of the enzyme in ...
M Y, Khan, S A, Newman
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1990
Abstract : Using rat serum as a source of esterase activity, we describe a quick and efficient method of taking advantage of the water solubility of the paranitrophenoxide anion. Esterase activity is terminated by adding chloroform and releasing paranitrophenol converted to the 400 nanometers absorbing paranitrophenoxide anion by adding 0.2 molar ...
James P. Chambers, James J. Valdes
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Abstract : Using rat serum as a source of esterase activity, we describe a quick and efficient method of taking advantage of the water solubility of the paranitrophenoxide anion. Esterase activity is terminated by adding chloroform and releasing paranitrophenol converted to the 400 nanometers absorbing paranitrophenoxide anion by adding 0.2 molar ...
James P. Chambers, James J. Valdes
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A colorimetric formaldehyde assay
Applied Biochemistry and Biotechnology, 1997A room-temperature assay of formaldehyde is described. The assay uses few reagents and is colorimetric, read at a wavelength of 649 nm. Tryptophan and tryptamine were noted as interfering with the assay, probably by binding with the formaldehyde. High levels of sugar show smaller effects on final absorbance. Glyceraldehyde also reacts in the assay, but
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Applied Biochemistry and Biotechnology, 1995
A new spectrophotometric method for measuring fructose is presented. The method uses Tryptamine in HCl acid, is carried out at 60°C, and is complete within 60 min. The assay is read at 518 nm and shows very low interference from other sugars. The method can be used for fructose, fructosans, and inulin.
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A new spectrophotometric method for measuring fructose is presented. The method uses Tryptamine in HCl acid, is carried out at 60°C, and is complete within 60 min. The assay is read at 518 nm and shows very low interference from other sugars. The method can be used for fructose, fructosans, and inulin.
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Colorimetric Assay of Amphotericin B
Journal of Pharmaceutical Sciences, 1963J C, CHANG +3 more
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Colorimetric assay for chitinase
1988Publisher Summary This chapter describes the colorimetric assay for chitinase, which is applicable to the various types of chitinase present in microorganisms, animals, and plants. The chapter evaluates it with particular reference to plant chitinases, because they may function as a defense against chitin-containing pathogens.
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Colorimetric Assays for Screening Laccases
2003Miguel, Alcalde, Thomas, Bulter
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