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Colorimetric Cotton Swab for Viral Protease Detection
Analytical Chemistry, 2022Reporting the activity of a specific viral protease remains an acute need for rapid point-of-care detection strategies that can distinguish active infection from a resolved infection. In this work, we present a simple colorimetric approach for reporting the activity of a specific viral protease through direct color conversion on a cotton swab, which ...
Ranit Dutta +8 more
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Colorimetric detection of acidic pesticides in water
Chemical Communications, 2022A water stable, porphyrin-based metal–organic framework as a colorimetric sensor for the detection of acidic pesticides.
Kyle T. Smith +4 more
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Polymer Micropillar Arrays for Colorimetric DNA Detection
Analytical Chemistry, 2020We describe the use of periodic micropillar arrays, produced from cyclic olefin copolymer using high-fidelity microfabrication, as templates for colorimetric DNA detection. The assay involves PCR-amplified gene markers for E. coli O157:H7 (rfbO157, eae, vt1, and vt2) incorporating a detectable digoxigenin label, which is revealed through an ...
Geissler, Matthias +8 more
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Quantitative PCR with colorimetric detection
Research in Immunology, 1996We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of ...
F, Demay +3 more
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Colorimetric Silver Detection of DNA Microarrays
Analytical Biochemistry, 2001Development of microarrays has revolutionized gene expression analysis and molecular diagnosis through miniaturization and the multiparametric features. Critical factors affecting detection efficiency of targets hybridization on microarray are the design of capture probes, the way they are attached to the support, and the sensitivity of the detection ...
Alexandre, Isabelle +7 more
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Detection of opines by colorimetric assay
Analytical Biochemistry, 1987A colorimetric procedure for confirming the presence of arginine-derived opines (nopaline and octopine) in plant tissue extracts is described. Those materials are widely used as markers of plant cell transformation and tumorigenesis mediated by the tumor-inducing plasmids of Agrobacterium tumefaciens.
N S, Yang, S G, Platt, P, Christou
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Nanozymatic Degradation and Simultaneous Colorimetric Detection of Tetracycline
Food Chemistry, 2022Tetracycline (TC) is a broad-spectrum antibiotic that can enter and accumulate in the human body via the food chain. Even in small concentrations, TC can cause several malignant health effects. We developed a system to simultaneously degrade the presence of TC in food matrices using titanium carbide MXene (FL-Ti3C2Tx).
Weizheng Wang +2 more
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Colorimetric-detected DNA sequencing
Analytical Biochemistry, 1987A sensitive, colorimetric method for visualizing the band pattern of DNA sequencing reaction is described. The enzymatic incorporation of radioactive nucleotides commonly used for the band detection is replaced by biotin conjugated to the 5'-terminus of a synthetic oligonucleotide by chemical synthesis.
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Colorimetric Detection of Acetyl Xylan Esterase Activities
2017Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates.
Mai-Gisondi, Galina, Master, Emma R.
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Labeling and Colorimetric Detection of Nonisotopic Probes
Current Protocols in Molecular Biology, 1992AbstractAlthough a number of different nonisotopic labels have been described in the literature, biotin and digoxigenin are used most frequently and are commercially available. Either label can be easily incorporated into DNA probes and be detected colorimetrically; a number of fluorochromes, as well as alkaline phosphatase and horseradish peroxidase ...
A, Boyle, H, Perry-O'Keefe
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