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Colorimetric Cotton Swab for Viral Protease Detection

Analytical Chemistry, 2022
Reporting the activity of a specific viral protease remains an acute need for rapid point-of-care detection strategies that can distinguish active infection from a resolved infection. In this work, we present a simple colorimetric approach for reporting the activity of a specific viral protease through direct color conversion on a cotton swab, which ...
Ranit Dutta   +8 more
openaire   +2 more sources

Colorimetric detection of acidic pesticides in water

Chemical Communications, 2022
A water stable, porphyrin-based metal–organic framework as a colorimetric sensor for the detection of acidic pesticides.
Kyle T. Smith   +4 more
openaire   +2 more sources

Polymer Micropillar Arrays for Colorimetric DNA Detection

Analytical Chemistry, 2020
We describe the use of periodic micropillar arrays, produced from cyclic olefin copolymer using high-fidelity microfabrication, as templates for colorimetric DNA detection. The assay involves PCR-amplified gene markers for E. coli O157:H7 (rfbO157, eae, vt1, and vt2) incorporating a detectable digoxigenin label, which is revealed through an ...
Geissler, Matthias   +8 more
openaire   +2 more sources

Quantitative PCR with colorimetric detection

Research in Immunology, 1996
We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of ...
F, Demay   +3 more
openaire   +2 more sources

Colorimetric Silver Detection of DNA Microarrays

Analytical Biochemistry, 2001
Development of microarrays has revolutionized gene expression analysis and molecular diagnosis through miniaturization and the multiparametric features. Critical factors affecting detection efficiency of targets hybridization on microarray are the design of capture probes, the way they are attached to the support, and the sensitivity of the detection ...
Alexandre, Isabelle   +7 more
openaire   +3 more sources

Detection of opines by colorimetric assay

Analytical Biochemistry, 1987
A colorimetric procedure for confirming the presence of arginine-derived opines (nopaline and octopine) in plant tissue extracts is described. Those materials are widely used as markers of plant cell transformation and tumorigenesis mediated by the tumor-inducing plasmids of Agrobacterium tumefaciens.
N S, Yang, S G, Platt, P, Christou
openaire   +2 more sources

Nanozymatic Degradation and Simultaneous Colorimetric Detection of Tetracycline

Food Chemistry, 2022
Tetracycline (TC) is a broad-spectrum antibiotic that can enter and accumulate in the human body via the food chain. Even in small concentrations, TC can cause several malignant health effects. We developed a system to simultaneously degrade the presence of TC in food matrices using titanium carbide MXene (FL-Ti3C2Tx).
Weizheng Wang   +2 more
openaire   +2 more sources

Colorimetric-detected DNA sequencing

Analytical Biochemistry, 1987
A sensitive, colorimetric method for visualizing the band pattern of DNA sequencing reaction is described. The enzymatic incorporation of radioactive nucleotides commonly used for the band detection is replaced by biotin conjugated to the 5'-terminus of a synthetic oligonucleotide by chemical synthesis.
openaire   +3 more sources

Colorimetric Detection of Acetyl Xylan Esterase Activities

2017
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates.
Mai-Gisondi, Galina, Master, Emma R.
openaire   +2 more sources

Labeling and Colorimetric Detection of Nonisotopic Probes

Current Protocols in Molecular Biology, 1992
AbstractAlthough a number of different nonisotopic labels have been described in the literature, biotin and digoxigenin are used most frequently and are commercially available. Either label can be easily incorporated into DNA probes and be detected colorimetrically; a number of fluorochromes, as well as alkaline phosphatase and horseradish peroxidase ...
A, Boyle, H, Perry-O'Keefe
openaire   +2 more sources

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